HA-tag Peptide PAT5E7AT Antibody

The HA-tag peptide Clone PAT5E7AT is a monoclonal antibody derived from mouse and is specifically designed to recognize the HA (hemagglutinin) tag. This tag is a short peptide sequence derived from the influenza hemagglutinin protein, commonly used in molecular biology to label and detect proteins.

The HA-tag was originally derived from the influenza virus hemagglutinin protein, specifically the sequence YPYDVPDYA. This sequence is recognized by specific antibodies, making it a useful tool for tagging proteins in various experimental setups. The Clone PAT5E7AT was developed by immunizing mice with a synthetic peptide containing the HA epitope, leading to the production of hybridoma cells that secrete the monoclonal antibody .

The HA-tag peptide Clone PAT5E7AT is widely used in various molecular biology techniques, including:

• Western Blotting: For detecting HA-tagged proteins in cell lysates.
• Immunoprecipitation: To isolate HA-tagged proteins from complex mixtures.
• Immunofluorescence: For visualizing the localization of HA-tagged proteins within cells.
• Flow Cytometry: To analyze the expression of HA-tagged proteins on the cell surface or within cells .

The Clone PAT5E7AT antibody is highly specific to the HA-tag and can recognize the tag fused to either the amino or carboxy terminus of target proteins. This specificity ensures minimal cross-reactivity with other proteins, making it a reliable tool for detecting HA-tagged proteins in various experimental conditions .

The antibody is typically supplied in a buffer containing sodium HEPES, NaCl, BSA, glycerol, and sodium azide. It should be stored at -20°C to maintain its stability and activity. It is important not to aliquot the antibody to avoid repeated freeze-thaw cycles, which can degrade its quality .