HA-HRP Antibody

The HA (Mouse Antibody, Peroxidase Conjugated) is a monoclonal antibody that specifically recognizes the HA-tag, a sequence derived from the human influenza hemagglutinin (HA) protein. This antibody is conjugated to peroxidase, an enzyme that catalyzes the oxidation of substrates by hydrogen peroxide, producing a detectable signal. The HA-tag is commonly used in molecular biology to facilitate the detection and purification of recombinant proteins.

The HA-tag consists of a nine-amino acid sequence (YPYDVPDYA) derived from the human influenza virus hemagglutinin protein, specifically from amino acids 98-106 . The HA (Mouse Antibody, Peroxidase Conjugated) is produced by immunizing mice with a synthetic peptide corresponding to this sequence. The resulting hybridoma cells are then fused with mouse myeloma cells to produce a stable cell line that secretes the monoclonal antibody .

The antibody is conjugated to horseradish peroxidase (HRP), an enzyme commonly used in biochemical assays due to its ability to produce a colorimetric, chemiluminescent, or fluorescent signal upon reaction with appropriate substrates . This conjugation enhances the antibody’s utility in various applications, including Western blotting, ELISA, and immunohistochemistry.

The HA (Mouse Antibody, Peroxidase Conjugated) is widely used in research for the detection of HA-tagged proteins. Its applications include:

• Western Blotting: Used to detect HA-tagged proteins separated by SDS-PAGE and transferred to a membrane .
• ELISA: Employed in enzyme-linked immunosorbent assays to quantify HA-tagged proteins in various samples .
• Immunohistochemistry: Utilized to visualize the localization of HA-tagged proteins in tissue sections .

The antibody is typically supplied in a lyophilized form and requires reconstitution with distilled water. It is recommended to store the reconstituted antibody at 2-8°C and avoid repeated freeze-thaw cycles to maintain its stability and activity .