HA Monoclonal Antibody

The HA (hemagglutinin) tag is a widely used epitope tag derived from the human influenza virus hemagglutinin protein. It is commonly used in molecular biology and biochemistry for the detection and purification of proteins. The HA tag sequence is YPYDVPDYA, and it can be fused to either the amino or carboxy terminus of a protein of interest .

The HA tag was first introduced in the late 1980s as a tool for studying protein expression and localization. It has since become a standard tool in many laboratories due to its small size, which minimizes interference with the protein’s function, and its high specificity and affinity for anti-HA antibodies .

Mouse monoclonal antibodies against the HA tag are commonly used in various applications, including:

• Western Blotting: To detect HA-tagged proteins in cell lysates.
• Immunoprecipitation: To isolate HA-tagged proteins from complex mixtures.
• Immunofluorescence: To visualize the localization of HA-tagged proteins within cells.
• Flow Cytometry: To analyze the expression of HA-tagged proteins on the cell surface .

Mouse monoclonal antibodies against the HA tag are highly specific and sensitive. They can detect HA-tagged proteins expressed at low levels and are compatible with various detection methods. These antibodies are produced by immunizing mice with a synthetic peptide containing the HA epitope, resulting in the generation of hybridoma cells that produce the desired antibody .

The HA tag and its corresponding mouse monoclonal antibodies have been instrumental in advancing our understanding of protein function and interactions. They are used in a wide range of research areas, including:

• Protein-Protein Interactions: To study the interactions between HA-tagged proteins and their binding partners.
• Protein Localization: To determine the subcellular localization of HA-tagged proteins.
• Protein Purification: To purify HA-tagged proteins for further biochemical analysis .