HA Antibody

HA Polyclonal Antibody is a widely used tool in molecular biology and biochemistry. It is designed to recognize and bind to the HA (hemagglutinin) tag, a short peptide sequence derived from the influenza virus hemagglutinin protein. This tag is often fused to proteins of interest to facilitate their detection, purification, and characterization.

The HA tag is a nine-amino acid sequence (YPYDVPDYA) that is commonly used as an epitope tag in various experimental applications. It is derived from the hemagglutinin protein of the influenza virus, which is a surface glycoprotein involved in the virus’s ability to infect host cells . The HA tag is typically fused to the N- or C-terminus of a protein of interest, allowing researchers to track and study the protein using anti-HA antibodies.

Polyclonal antibodies (PAbs) are a mixture of antibodies produced by different B cell lineages within the body. Each antibody in the mixture recognizes a different epitope on the same antigen, providing a robust and versatile tool for detecting proteins . Polyclonal antibodies are generated by immunizing an animal (such as a rabbit) with an antigen, in this case, the HA tag. The animal’s immune system produces a diverse array of antibodies against the antigen, which are then collected and purified from the animal’s serum.

To produce HA Polyclonal Antibody, a synthetic peptide corresponding to the HA tag sequence is used as the immunogen. This peptide is injected into an animal, typically a rabbit, to elicit an immune response. Over time, the animal produces antibodies against the HA tag, which are then harvested from the animal’s blood. The antibodies are purified using antigen affinity chromatography, ensuring that the final product is highly specific to the HA tag .

HA Polyclonal Antibody is used in a variety of experimental techniques, including:

• Western Blotting: To detect HA-tagged proteins in cell lysates or tissue extracts.
• Immunoprecipitation: To isolate HA-tagged proteins and their interacting partners from complex mixtures.
• Immunofluorescence: To visualize the localization of HA-tagged proteins within cells.
• ELISA: To quantify the amount of HA-tagged protein in a sample.
• Flow Cytometry: To analyze the expression of HA-tagged proteins on the surface of cells .

The use of HA Polyclonal Antibody offers several advantages:

• High Sensitivity: Polyclonal antibodies can recognize multiple epitopes on the HA tag, increasing the likelihood of detection.
• Versatility: Suitable for various applications, including Western blotting, immunoprecipitation, and immunofluorescence.
• Specificity: The antibodies are highly specific to the HA tag, minimizing cross-reactivity with other proteins.