GSTM1 Mouse

Glutathione S-Transferase M1 (GSTM1) is a member of the glutathione S-transferase (GST) family, which plays a crucial role in detoxification processes. GSTs are enzymes that catalyze the conjugation of the antioxidant molecule glutathione (GSH) to various endogenous and exogenous electrophilic compounds. This process is essential for the detoxification of harmful substances and the protection of cellular components from oxidative damage.

GSTM1 is a phase II detoxification enzyme predominantly expressed in the liver. It is involved in the metabolism of a wide range of substrates, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. The enzyme’s activity is crucial for maintaining cellular homeostasis and protecting against cellular damage.

The structure of GSTM1 includes a highly conserved GSH-binding site and a more variable substrate-binding site. This dual binding capability allows GSTM1 to interact with a diverse array of substrates, making it a versatile enzyme in detoxification pathways.

Recombinant GSTM1 (Mouse) is produced using Escherichia coli expression systems. The recombinant protein is typically purified to a high degree of purity (>95%) and is suitable for various biochemical assays, including SDS-PAGE. The availability of recombinant GSTM1 allows for detailed studies on its enzymatic properties, substrate specificity, and potential applications in biotechnology and medicine .

GSTM1 plays a significant role in protecting cells from oxidative stress and toxic insults. It is involved in the detoxification of reactive oxygen species (ROS) and the metabolism of xenobiotics. The enzyme’s activity is particularly important in the liver, where it helps to neutralize harmful compounds and prevent liver damage.

Research has shown that GSTM1 is upregulated in response to chronic alcohol consumption, providing a protective mechanism against alcohol-induced liver injury. The enzyme counteracts the activation of apoptosis signal-regulating kinase 1 (ASK1) and its downstream signaling pathways, thereby mitigating oxidative stress and inflammation in the liver .

Recombinant GSTM1 is widely used in research to study its role in detoxification processes and its potential therapeutic applications. The enzyme’s ability to neutralize harmful compounds makes it a valuable tool in the development of treatments for diseases associated with oxidative stress and toxin exposure.

In addition, GSTM1 has potential applications in biotechnology, such as the development of biosensors for detecting environmental toxins and the engineering of plants with enhanced resistance to biotic and abiotic stresses .