GSTM1 Mouse, His
Description
Product Specs
MGSSHHHHHH SSGLVPRGSH MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS SRYIATPIFS KMAHWSNK.
Product Science Overview
GSTM1 is one of the isoforms of GST and is encoded by the GSTM1 gene. This enzyme is particularly important in the metabolism of carcinogens, drugs, and products of oxidative stress. The GSTM1 gene is polymorphic, meaning that there are variations in the gene sequence among individuals, which can affect the enzyme’s activity and, consequently, an individual’s susceptibility to certain diseases.
Recombinant GSTM1 refers to the enzyme produced through recombinant DNA technology. This involves inserting the GSTM1 gene into an expression vector, which is then introduced into a host organism, such as E. coli, to produce the enzyme in large quantities. The recombinant enzyme can be tagged with a histidine tag (His Tag) to facilitate its purification and detection.
A His Tag is a string of histidine residues (usually six) added to either the N- or C-terminus of a protein. This tag allows for easy purification of the recombinant protein using metal affinity chromatography, as histidine residues have a high affinity for divalent metal ions like nickel or cobalt. The His Tag also aids in the detection of the protein using anti-His antibodies.
The production of recombinant GSTM1 involves cloning the GSTM1 gene into an expression vector that includes a His Tag sequence. The vector is then introduced into a host organism, such as E. coli, where the recombinant protein is expressed. The His Tag allows for the purification of the protein using nickel or cobalt affinity chromatography .
Recombinant GSTM1 with a His Tag is widely used in research to study the enzyme’s function, structure, and interactions with other molecules. It is also used in drug development to screen for potential inhibitors of GSTM1, which could be used to enhance the efficacy of chemotherapy drugs by preventing the detoxification of these drugs by GSTM1.
