Introduction
Glutathione S-transferase zeta 1 (GSTZ1) is a member of the glutathione S-transferase superfamily, which consists of multifunctional enzymes crucial for detoxifying electrophilic compounds. These compounds, including carcinogens, mutagens, and various therapeutic drugs, are neutralized through conjugation with glutathione. GSTZ1 plays a vital role in the catabolism of phenylalanine and tyrosine. Consequently, defects in GSTZ1 can lead to severe metabolic disorders such as alkaptonuria, phenylketonuria, and tyrosinemia. Functionally, GSTZ1 exhibits bifunctional properties with minimal glutathione-conjugating activity towards 7-chloro-4-nitrobenz-2-oxa-1,3-diazole and possesses maleylacetoacetate isomerase activity. Additionally, it demonstrates low glutathione peroxidase activity with T-butyl and cumene hydroperoxides. GSTZ1 catalyzes the glutathione-dependent oxygenation of dichloroacetic acid to glyoxylic acid.
Description
Recombinant human GSTZ1, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 236 amino acids (1-216 a.a.) with a molecular weight of 26.2 kDa. The protein is engineered with a 20 amino acid His-Tag at the N-terminus and purified using conventional chromatography techniques.
Physical Appearance
Colorless, sterile-filtered solution.
Formulation
The GSTZ1 protein solution is supplied in 1x PBS (pH 7.4) with 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), store at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity greater than 95.0% as determined by SDS-PAGE analysis.
Synonyms
MAAI, GSTZ-1, MAI, Maleylacetone Isomerase, EC 2.5.1.18, Maleylacetoacetate isomerase, Glutathione S-transferase zeta 1, EC 5.2.1.2, GSTZ1-1, MGC2029, GSTZ1.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MQAGKPILYS YFRSSCSWRV RIALALKGID YETVPINLIK DGGQQFSKDF QALNPMKQVP TLKIDGITIH QSLAIIEYLE ETRPTPRLLP QDPKKRASVR MISDLIAGGI QPLQNLSVLK QVGEEMQLTW AQNAITCGFN ALEQILQSTA GIYCVGDEVT MADLCLVPQV ANAERFKVDL TPYPTISSIN KRLLVLEAFQ VSHPCRQPDT PTELRA.
