GSTM1 Human

Glutathione S-Transferase M1 Human Recombinant
Cat. No.
BT6914
Source
Escherichia Coli.
Synonyms
GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, MU, MU-1, GST HB subunit 4, GST class-mu 1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GSTM1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 203 amino acids (1-181 a.a) and having a molecular mass of 23.6kDa.GSTM1 is fused to a 22 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Glutathione S-transferases (GSTs) are a family of enzymes that play a critical role in detoxification by catalyzing the conjugation of glutathione to a wide range of electrophilic substrates. These substrates include carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. Among the various GST classes, the mu class, encoded by genes clustered on chromosome 1p13.3, exhibits significant polymorphism, influencing individual susceptibility to carcinogens and toxins, as well as the effectiveness and potential toxicity of certain drugs. Notably, null mutations in the mu class gene have been implicated in an elevated risk of several cancers.
Description
Recombinant human GSTM1 protein, expressed in E. coli, is available as a single, non-glycosylated polypeptide chain. The protein consists of 203 amino acids, with amino acids 1-181 representing the GSTM1 sequence, followed by a 22 amino acid His-tag at the N-terminus. The molecular weight of the protein is 23.6 kDa. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
The product is a clear, sterile-filtered solution.
Formulation
The GSTM1 protein solution is provided at a concentration of 0.25 mg/ml in a buffer containing 20mM Tris-HCl (pH 8.0), 0.4M Urea, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. It is crucial to avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of the GSTM1 protein is determined to be greater than 85.0% using SDS-PAGE analysis.
Synonyms
GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, MU, MU-1, GST HB subunit 4, GST class-mu 1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH RSMPMILGYW DIRGLAHAIR LLLEYTDSSY EEKKYTMGDA PDYDRSQWLN EKFKLGLDFP NLPYLIDGAH KITQSNAILC YIARKHNLCG ETEEEKIRVD ILENQTMDNH MQLGMICYNP EFEKLKPKYL EELPEKLKLY SEFLGKRPWF AGNKGLEKIS AYMKSSRFLP RPVFSKMAVW GNK.

Product Science Overview

Structure and Function

GSTM1 is encoded by the GSTM1 gene, which is located on chromosome 1p13.3. The enzyme has a molecular weight of approximately 26 kDa and is expressed in various tissues, including the liver, kidney, and lungs . GSTM1 functions by binding to glutathione and facilitating the conjugation of glutathione with toxic substances, making them more water-soluble and easier to excrete from the body .

Recombinant GSTM1

Recombinant GSTM1 is produced using Escherichia coli (E. coli) expression systems. The recombinant form is often used in research and industrial applications due to its high purity and specific activity. The enzyme is typically stored as a frozen liquid at -70°C to maintain its stability and activity .

Biological Significance

GSTM1 plays a significant role in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress . The enzyme’s activity helps protect cells from damage caused by these harmful substances. Additionally, GSTM1 is involved in the metabolism of xenobiotics and contributes to the body’s defense against oxidative stress .

Genetic Polymorphisms and Health Implications

The GSTM1 gene is highly polymorphic, with several genetic variations that can influence an individual’s susceptibility to toxins and carcinogens. One notable polymorphism is the GSTM1 null genotype, where the gene is deleted. Individuals with this genotype lack GSTM1 enzyme activity, which has been associated with an increased risk of developing certain cancers and other diseases due to reduced detoxification capacity .

Applications in Research and Medicine

Recombinant GSTM1 is widely used in biochemical research to study the enzyme’s structure, function, and role in detoxification processes. It is also utilized in drug development and toxicology studies to evaluate the effects of various compounds on GSTM1 activity. Furthermore, understanding the genetic variations of GSTM1 can aid in personalized medicine approaches, where treatments are tailored based on an individual’s genetic makeup .

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