GH1 Antibody
Description
Product Specs
Product Science Overview
Growth Hormone-1 (GH1), also known as somatotropin, is a protein hormone that plays a crucial role in growth, metabolism, and overall development in humans. The mouse anti-human GH1 antibody is a monoclonal antibody specifically designed to target and bind to human GH1. This antibody is widely used in various scientific applications, including Western Blot (WB), Immunohistochemistry (IHC), Enzyme-Linked Immunosorbent Assay (ELISA), and Immunoprecipitation (IP).
GH1 is a single-chain polypeptide consisting of 191 amino acids. It is produced by the somatotropic cells of the anterior pituitary gland. The primary function of GH1 is to stimulate growth and cell reproduction. It exerts its effects by binding to the growth hormone receptor (GHR) on the surface of target cells, leading to the activation of the JAK-STAT signaling pathway. This activation results in the production of insulin-like growth factor 1 (IGF-1), which mediates many of the growth-promoting effects of GH1.
The mouse anti-human GH1 antibody is produced using hybridoma technology. This involves the fusion of a mouse myeloma cell with a B cell obtained from a mouse immunized with purified, recombinant human GH1. The resulting hybridoma cells are capable of producing large quantities of monoclonal antibodies specific to human GH1.
- Western Blot (WB): The mouse anti-human GH1 antibody is commonly used in Western Blot to detect the presence of GH1 in various biological samples. It binds specifically to GH1, allowing for the visualization of the protein on a membrane after electrophoresis.
- Immunohistochemistry (IHC): In IHC, this antibody is used to detect GH1 in tissue sections. It helps in studying the distribution and localization of GH1 within different tissues.
- Enzyme-Linked Immunosorbent Assay (ELISA): The antibody is used in ELISA to quantify the levels of GH1 in serum, plasma, or other biological fluids. This is particularly useful in clinical diagnostics and research studies.
- Immunoprecipitation (IP): The antibody can be used to isolate GH1 from a mixture of proteins, allowing for further analysis of the protein and its interactions.
The mouse anti-human GH1 antibody is a valuable tool in both basic and clinical research. It aids in understanding the role of GH1 in growth and development, as well as its involvement in various diseases and disorders. For instance, abnormalities in GH1 levels are associated with conditions such as gigantism, acromegaly, and growth hormone deficiency. By using this antibody, researchers can investigate the underlying mechanisms of these conditions and develop potential therapeutic strategies.
