HGH Antibody
Description
Product Specs
Product Science Overview
Growth hormone (GH), also known as somatotropin, is a peptide hormone that stimulates growth, cell reproduction, and cell regeneration in humans and other animals. It is crucial for human development and plays a significant role in maintaining tissue and organ health throughout life. The growth hormone polyclonal rabbit anti-human antibody is a vital tool in biomedical research, particularly in the study of growth hormone-related functions and disorders.
Polyclonal antibodies are produced by immunizing an animal, in this case, a rabbit, with an antigen—in this context, human growth hormone. The rabbit’s immune system recognizes the human growth hormone as foreign and generates a diverse array of antibodies against it. These antibodies are then harvested from the rabbit’s serum.
The polyclonal nature of these antibodies means they consist of a mixture of immunoglobulin molecules that recognize multiple epitopes on the target antigen. This diversity can be advantageous in certain applications, as it increases the likelihood of detecting the antigen under various conditions.
The growth hormone polyclonal rabbit anti-human antibody is used in several scientific applications, including:
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Immunohistochemistry (IHC): This technique involves staining tissue sections to visualize the presence and localization of growth hormone within the tissues. It is particularly useful in studying the distribution and expression patterns of growth hormone in different tissues and under various physiological and pathological conditions .
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Western Blotting: This method is used to detect specific proteins in a sample. The antibody binds to the growth hormone, allowing researchers to identify and quantify the hormone in different samples .
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Enzyme-Linked Immunosorbent Assay (ELISA): This technique is used to measure the concentration of growth hormone in various biological samples. The polyclonal antibody’s ability to recognize multiple epitopes enhances the sensitivity and specificity of the assay .
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Immunoprecipitation: This method is used to isolate and concentrate growth hormone from a mixture of proteins. The polyclonal antibody binds to the growth hormone, allowing it to be separated from other proteins in the sample .
Advantages:
- High Sensitivity: The ability to recognize multiple epitopes increases the likelihood of detecting the antigen.
- Versatility: Suitable for various applications, including IHC, Western blotting, ELISA, and immunoprecipitation.
- Cost-Effective: Generally less expensive to produce compared to monoclonal antibodies.
Limitations:
- Batch Variability: Different batches of polyclonal antibodies can vary in their specificity and affinity, leading to potential inconsistencies in experimental results.
- Cross-Reactivity: The presence of multiple antibodies increases the risk of cross-reactivity with other proteins, which can lead to non-specific binding and false-positive results.
