GH Ovine, Placental
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Description
The GH Ovine Recombinant is purified by proprietary chromatographic techniques.
Placental Growth Hormone differs from pituitary Ovine Growth Hormone by two amino acids G9R/G63S. Placental Ovine Growth Hormone possesses higher biological activity as compared to pituitary Ovine GH.
Product Specs
Product Science Overview
Placental Ovine Growth Hormone Recombinant (POGH-R) is a synthetic version of the naturally occurring growth hormone found in sheep (ovine). This hormone is part of the somatotropin/prolactin family, which plays a crucial role in growth regulation and metabolic processes. The recombinant form is produced using advanced biotechnological methods, typically involving the expression of the hormone in bacterial systems such as Escherichia coli .
POGH-R is a single, non-glycosylated polypeptide chain consisting of 191 amino acids, with a molecular mass of approximately 21,918 Daltons . The production process involves the insertion of the ovine growth hormone gene into a bacterial plasmid, which is then introduced into E. coli. The bacteria express the hormone, which is subsequently purified using proprietary chromatographic techniques .
The biological activity of POGH-R is significant, as it is fully active when compared to the World Health Organization (WHO) reference standard. It is capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD), indicating its functional similarity to the natural hormone . POGH-R has been shown to possess higher biological activity compared to pituitary-derived ovine growth hormone, due to differences in amino acid sequences .
POGH-R is used extensively in research to study the effects of growth hormones on various physiological processes. It has been employed in studies investigating endometrial function, where it was found to regulate endometrial gland proliferation and differentiation . Additionally, POGH-R is used in agricultural practices to enhance growth rates and improve the overall health of livestock.
The stability of POGH-R is noteworthy. When lyophilized, it remains stable at room temperature for up to three weeks. For long-term storage, it is recommended to keep the hormone desiccated below -18°C. Upon reconstitution, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term use. To prevent degradation, it is advisable to add a carrier protein such as human serum albumin (HSA) or bovine serum albumin (BSA) and avoid freeze-thaw cycles .
