GH Antagonist Ovine

Growth Hormone Antagonist Ovine Recombinant
Cat. No.
BT11783
Source
Escherichia Coli.
Synonyms
GH1, GH, GHN, GH-N, hGH-N, Pituitary growth hormone, Growth hormone 1, Somatotropin.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC
(b) Analysis by Gel Filtration. (c) Analysis by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Somatotropin Ovine Antagonist Recombinant G119R produced in E.Coli is a single, non-glycosylated polypeptide chain containing 191 amino acids and having a molecular mass of 22 kDa.
The Somatotropin Ovine Antagonist Recombinant is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Growth Hormone (GH) is a member of the somatotropin/prolactin family of hormones that play a crucial role in regulating growth. The GH gene, along with four other related genes, is located within the growth hormone locus on chromosome 17. These genes are arranged in the same transcriptional orientation, suggesting an evolutionary history of gene duplication. Notably, the five genes exhibit a high degree of sequence identity. Alternative splicing further contributes to the diversity of growth hormones by generating additional isoforms, potentially leading to functional specialization. This specific family member is expressed in the pituitary gland but not in placental tissue, unlike the other four genes in the growth hormone locus. Mutations or deletions affecting this gene can result in growth hormone deficiency and short stature.
Description
Somatotropin Ovine Antagonist Recombinant G119R, produced in E. coli, is a single, non-glycosylated polypeptide chain composed of 191 amino acids, with a molecular weight of 22 kDa. The purification of this recombinant antagonist is achieved through proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The protein was lyophilized from a concentrated solution (1mg/ml) containing 0.0045M NaHCO3, at a pH of 9.
Solubility
For reconstitution of lyophilized GH G119R, it is recommended to use 0.4% NaHCO3 or water adjusted to a pH of 9. The initial reconstitution concentration should be at least 100 µg/ml. Following this, further dilutions can be made using other aqueous solutions, preferably with the addition of a carrier protein such as BSA or a similar agent.
Stability
Lyophilized GH G119R exhibits stability at room temperature for a minimum of two weeks. However, it is recommended to store the lyophilized powder desiccated at a temperature below -18°C. Once reconstituted and sterilized by filtration, GH can be stored at 4°C and pH 9 for up to 4 weeks. For long-term storage or when handling more diluted solutions, adding a carrier protein (0.1% HSA or BSA) is advised. It is important to avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 98.0% using the following methods: (a) Analysis by RP-HPLC, (b) Analysis by Gel Filtration, and (c) Analysis by SDS-PAGE.
Biological Activity
GH G119R functions as an antagonist, as demonstrated in an in vitro bioassay using PDF-P1 3B9 cells stably transfected with rabbit GH receptors. It forms a 1:1 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD) and binds to this ECD with an affinity comparable to that of the wild-type oGH.
Protein Content
Protein quantification was performed using UV spectroscopy at 280 nm. An absorbance value of 0.63 was used as the extinction coefficient for a 0.1% (1mg/ml) solution. This value was calculated using the DNAman computer analysis program based on protein sequences.
Synonyms
GH1, GH, GHN, GH-N, hGH-N, Pituitary growth hormone, Growth hormone 1, Somatotropin.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala.

Product Science Overview

Introduction

Growth Hormone Antagonist Ovine Recombinant (roGH-A) is a synthetic protein designed to inhibit the activity of growth hormone (GH). This antagonist is derived from ovine (sheep) growth hormone and is produced using recombinant DNA technology. The primary purpose of this antagonist is to study and potentially treat conditions related to excessive growth hormone activity.

Growth Hormone and Its Functions

Growth hormone, also known as somatotropin, is a protein hormone produced by the pituitary gland. It plays a crucial role in growth, metabolism, and cell regeneration. GH stimulates growth in almost all tissues of the body, including bones and muscles, by promoting protein synthesis and increasing the rate of cell division.

Recombinant DNA Technology

Recombinant DNA technology involves combining DNA from different sources to create a new genetic sequence. This technology is used to produce recombinant proteins, such as roGH-A, by inserting the gene encoding the desired protein into a host organism, typically bacteria or yeast. The host organism then produces the protein, which can be purified and used for research or therapeutic purposes.

Mechanism of Action

The growth hormone antagonist works by binding to the growth hormone receptor (GHR) without activating it. This prevents the natural growth hormone from binding to the receptor and exerting its effects. By blocking the receptor, the antagonist effectively reduces the biological activity of growth hormone. This mechanism is particularly useful in studying the role of GH in various physiological processes and in developing treatments for conditions caused by excessive GH activity.

Applications and Research
  1. Acromegaly Treatment: One of the primary applications of growth hormone antagonists is in the treatment of acromegaly, a condition characterized by excessive growth hormone production. Acromegaly leads to abnormal growth of bones and tissues, resulting in enlarged hands, feet, and facial features. By inhibiting GH activity, roGH-A can help manage the symptoms of acromegaly.
  2. Cancer Research: Growth hormone antagonists are also being investigated for their potential role in cancer treatment. Some cancers are known to be influenced by growth hormone, and inhibiting GH activity may help slow down the progression of these cancers.
  3. Metabolic Studies: Researchers use growth hormone antagonists to study the metabolic effects of GH. By blocking GH activity, scientists can better understand its role in metabolism, including its effects on glucose and lipid metabolism.

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