GH Antagonist Chicken

Growth Hormone Antagonist Chicken Recombinant
Cat. No.
BT11714
Source
Escherichia Coli.
Synonyms
GH1, GH, GHN, GH-N, hGH-N, Pituitary growth hormone, Growth hormone 1, Somatotropin.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

Somatotropin Chicken Antagonist Recombinant mutein G119R produced in E.Coli is a single, non-glycosylated polypeptide chain containing 191 amino acids with an additional Ala at the N-terminus and having a molecular mass of 22.3 kDa.
The Chicken Growth-Hormone Antagonist Recombinant is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Growth hormone (GH) is part of the somatotropin/prolactin hormone family, essential for growth regulation. This gene, along with four others, forms the growth hormone locus on chromosome 17. They share the same transcriptional orientation, suggesting evolution through gene duplication. These five genes exhibit significant sequence similarity. Alternative splicing further diversifies them, creating additional isoforms and potential specialization. Unlike the other four genes in the locus, this specific member expresses in the pituitary but not placental tissue. Mutations or deletions in this gene cause growth hormone deficiency, leading to short stature.
Description
Somatotropin Chicken Antagonist Recombinant mutein G119R, produced in E. coli, is a single, non-glycosylated polypeptide chain. It contains 191 amino acids, including an additional Alanine at the N-terminus, and has a molecular mass of 22.3 kDa. The purification of the Chicken Growth-Hormone Antagonist Recombinant is achieved using proprietary chromatographic techniques.
Physical Appearance
The product appears as a sterile, filtered white lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution containing 0.3% NaHCO3 at a pH of 8.
Solubility
For reconstitution of the lyophilized Growth-Hormone Chicken antagonist, it is recommended to use 0.4% NaHCO3 or water adjusted to a pH of 8-9. The concentration should be at least 100 µg/ml. This solution can be further diluted into other aqueous solutions, ideally with a carrier protein like BSA or similar.
Stability
Lyophilized Growth-Hormone Chicken antagonist remains stable at room temperature for a minimum of two weeks. However, for storage, it is recommended to keep it desiccated below -18°C. Once reconstituted and filter-sterilized, GH remains stable at 4°C for up to 4 weeks at a pH of 9. For long-term storage and in diluted solutions, adding a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 98.0% using the following methods: (a) SEC-HPLC analysis and (b) SDS-PAGE analysis.
Biological Activity
Recombinant Chicken Growth Hormone G119R mutant demonstrated no binding to ovine GHR-ECD and showed no biological activity in FDC-P1 3B9 cells. However, in binding experiments using chicken liver membranes, both ovine GH and chicken GH exhibited similar IC50 values when competing with 125I-ovine GH. The G119R mutein had a 10-fold higher IC50. These findings highlight the significance of species specificity and suggest the potential antagonistic activity of chGH G119R in homologous systems.
Protein Content
Protein quantification was performed using UV spectroscopy at 280 nm. An absorbency value of 0.8 was used as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program, which analyzes protein sequences (IntelliGenetics).
Synonyms
GH1, GH, GHN, GH-N, hGH-N, Pituitary growth hormone, Growth hormone 1, Somatotropin.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala.

Product Science Overview

Introduction

Growth Hormone Antagonist (Chicken Recombinant) is a recombinant protein derived from chicken growth hormone. It is a mutein, specifically the G119R mutant, produced in Escherichia coli (E. coli). This antagonist is a single, non-glycosylated polypeptide chain containing 191 amino acids with an additional alanine at the N-terminus, resulting in a molecular mass of approximately 22.3 kDa .

Structure and Production

The recombinant chicken growth hormone antagonist is produced using proprietary chromatographic techniques to ensure high purity. The protein is typically lyophilized (freeze-dried) into a sterile, white powder. The lyophilized form is stable at room temperature for at least two weeks but should be stored desiccated below -18°C for long-term stability. Upon reconstitution and filter sterilization, it can be stored at 4°C, pH 9, for up to four weeks .

Biological Activity

The G119R mutant of chicken growth hormone does not bind to the ovine growth hormone receptor extracellular domain (GHR-ECD) and lacks biological activity in FDC-P1 3B9 cells. However, in binding experiments using chicken liver membranes, both ovine growth hormone and chicken growth hormone showed similar IC50 values in competition with 125I-ovine growth hormone. The IC50 of the G119R mutein was found to be tenfold higher .

Applications

Growth hormone antagonists are valuable tools in research for studying growth hormone function and regulation. They can be used to investigate the mechanisms of growth hormone action, receptor binding, and signal transduction pathways. Additionally, they may have potential therapeutic applications in conditions where growth hormone activity needs to be modulated .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

HGH Antibody
Growth Hormone Polyclonal Rabbit Anti Human Antibody
Cat. No.
BT5538
Source
GH Antagonist Ovine
Growth Hormone Antagonist Ovine Recombinant
Cat. No.
BT11783
Source
Escherichia Coli.
GH Rainbow Trout
Growth Hormone Rainbow Trout (Oncorhynchus mykiss) Recombinant
Cat. No.
BT14028
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.