GH Denis

GH Denis Recombinant
Cat. No.
BT12865
Source
Escherichia Coli.
Synonyms

GH1, GH, GHN, GH-N, hGH-N,Pituitary GH, GH 1.

Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

GH Denis Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 190 amino acids and having a molecular mass of 21810 Dalton.
GH is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Growth Hormone (GH) belongs to the prolactin family of hormones, which are crucial for growth regulation. The GH gene, along with four related genes, forms the GH locus on chromosome 17. These genes share the same transcriptional orientation, suggesting evolution through gene duplication. A high degree of sequence identity exists among the five genes. Alternative splicing further diversifies the five GHs by generating additional isoforms, potentially leading to specialized functions. This specific family member is expressed in the pituitary gland but not in placental tissue, unlike the other four genes in the GH locus. Mutations or deletions in this gene can result in GH deficiency, leading to short stature.
Description
Recombinant GH Denis, produced in E. coli, is a single, non-glycosylated polypeptide chain composed of 190 amino acids. It has a molecular weight of 21.81 kDa. The purification process of GH involves proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder
Formulation
The protein was lyophilized from a 1 mg/ml solution containing 0.0045 mM NaHCO3.
Solubility
Reconstitute the lyophilized GH Denis in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted into other aqueous solutions as needed.
Stability
Lyophilized GH Denis remains stable at room temperature for up to 3 weeks. However, for long-term storage, it should be kept desiccated at a temperature below -18°C. After reconstitution, GH Denis should be stored at 4°C for 2-7 days. For longer storage periods, freeze at -18°C. To enhance stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
The purity of GH Denis is greater than 97.0%, as determined by the following methods: (a) Size Exclusion Chromatography - High Performance Liquid Chromatography (SEC-HPLC) (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)
Synonyms

GH1, GH, GHN, GH-N, hGH-N,Pituitary GH, GH 1.

Source
Escherichia Coli.

Product Science Overview

Introduction

Growth hormone (GH) is a member of the prolactin family of hormones, which play a crucial role in growth control. The gene responsible for GH, along with four other related genes, is located at the GH locus on chromosome 17. These genes share a high degree of sequence identity, and alternative splicing generates additional isoforms, leading to further diversity and specialization. GH Denis Recombinant is expressed in the pituitary gland but not in placental tissue, unlike the other genes in the GH locus .

Production

GH Denis Recombinant is produced in Escherichia coli (E. coli) bacteria. The production process involves inserting the gene encoding the human growth hormone into the bacterial DNA. The bacteria then use this genetic information to produce the GH protein. The resulting protein is a single, non-glycosylated polypeptide chain containing 190 amino acids and has a molecular mass of 21,810 Daltons .

The protein is purified using proprietary chromatographic techniques to ensure high purity and quality. The final product is a sterile, filtered, white lyophilized (freeze-dried) powder .

Stability and Storage

Lyophilized GH Denis Recombinant is stable at room temperature for up to three weeks. However, for long-term storage, it should be kept desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term use. It is recommended to add a carrier protein, such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA), to prevent freeze-thaw cycles .

Applications

GH Denis Recombinant is primarily used for laboratory research purposes. It is not intended for use as a drug, agricultural or pesticidal product, food additive, or household chemical .

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