GH Chicken
GH1, GH, GHN, GH-N, hGH-N,Pituitary GH, GH 1,
(a) Analysis by SDS-PAGE gel.
(b) Analysis by SEC-HPLC.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY.They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
Description
GH Chicken Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids with an additional Ala at its N-terminus and having a molecular mass of 22255 Dalton.
GH Chicken recombinant is purified by proprietary chromatographic techniques.
Product Specs
Growth hormone (GH) is a member of the prolactin family of hormones and plays a crucial role in regulating growth. The GH gene, along with four related genes, forms the GH locus on chromosome 17. These genes share the same transcriptional orientation, suggesting their origin from gene duplication events. The five genes exhibit a high degree of sequence similarity. Alternative splicing further diversifies the GH isoforms, potentially leading to functional specialization. This specific family member is expressed in the pituitary gland but not in placental tissue, unlike the other four genes in the GH locus. Mutations or deletions in this gene result in GH deficiency, leading to short stature.
Recombinant Chicken GH, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 191 amino acids. It includes an additional Alanine residue at the N-terminus, resulting in a molecular weight of 22255 Daltons. Purification of recombinant Chicken GH is achieved using proprietary chromatographic techniques.
(a) SDS-PAGE gel analysis.
(b) SEC-HPLC analysis.
Protein quantification was performed using two independent methods:
1. UV spectroscopy at 280 nm, employing an absorbance value of 0.75 as the extinction coefficient for a 0.1% (1 mg/ml) solution at pH 8.0. This value is derived from the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. RP-HPLC analysis, utilizing a calibrated GH solution as a reference standard.
GH1, GH, GHN, GH-N, hGH-N,Pituitary GH, GH 1,
Product Science Overview
The rcGH is a single, non-glycosylated polypeptide chain consisting of 191 amino acids with an additional alanine at its N-terminus. It has a molecular mass of approximately 22,255 Daltons . The recombinant hormone is biologically active and can mimic the effects of natural GH in homologous assays .
The production and release of GH are regulated by the hypothalamus and pituitary gland through a feedback loop involving growth hormone-releasing hormone (GHRH) and somatostatin. Recombinant GH, when administered exogenously, can bypass these regulatory mechanisms and directly influence growth and metabolism .
Recombinant chicken GH has been studied for its potential to enhance growth and feed efficiency in broiler chickens. However, studies have shown that while rcGH can induce significant metabolic effects, it may not necessarily lead to increased growth in highly selected poultry breeds . Nonetheless, rcGH remains a valuable tool for research in endocrinology and poultry science.
