GPT2 Mouse

Glutamic-Pyruvate Transaminase 2 Mouse Recombinant
Cat. No.
BT1735
Source
E.coli.
Synonyms
ALT2, AAT2, Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2.
Appearance
Sterile Filtered colorless solution.
Purity

Greater than 85% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GPT2 Mouse Recombinant produced in E. coli is a single polypeptide chain containing 522 amino acids (1-522) and having a molecular mass of 60.1 kDa. Mouse GPT2 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Glutamic-Pyruvate Transaminase 2 (GPT2), also known as alanine transaminase 2, is an enzyme that plays a crucial role in the metabolism of glucose and amino acids. Primarily found in muscle, fat, and kidney tissues, GPT2 catalyzes the reversible transfer of an amino group between alanine and 2-oxoglutarate, resulting in the formation of pyruvate and glutamate.
Description
This product consists of recombinant Mouse GPT2, a single polypeptide chain with a molecular weight of 60.1 kDa. It is produced in E. coli and encompasses amino acids 1-522 of the native GPT2 sequence. For purification and detection purposes, a 21 amino acid His-tag is fused to the N-terminus. The recombinant protein has been purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The provided GPT2 solution has a concentration of 1mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 7.5), 20% glycerol, and 2mM DTT.
Stability
For short-term storage (up to 4 weeks), keep refrigerated at 4°C. For extended storage, freeze the solution at -20°C. Adding a carrier protein such as HSA or BSA to a final concentration of 0.1% is recommended for long-term storage. Avoid repeated freeze-thaw cycles to preserve enzyme activity.
Purity
The purity of this product is greater than 85% as determined by SDS-PAGE analysis.
Biological Activity
This GPT2 enzyme exhibits high biological activity, exceeding 50 units/mg. One unit of activity is defined as the amount of enzyme required to catalyze the conversion of 1 μmole of L-Alanine to L-Glutamate per minute at 37°C and pH 7.5.
Synonyms
ALT2, AAT2, Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2.
Source
E.coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MQRAAVLVRR GSCPRASGPW GRSHSSAAAE ASAALKVRPE RSPRDRILTL ESMNPQVKAV EYAVRGPIVL KAGEIEMELQ RGIKKPFTEV IRANIGDAHA MGQQPITFLR QVMALCTYPN LLNSPSFPED AKKRARRILQ ACGGNSLGSY SASQGVNCIR EDVAAFITRR DGVPADPDNI YLTTGASDGI STILKLLVSG GGKSRTGVMI PIPQYPLYSA VISELDAVQV NYYLDEENCW ALNVDELRRA LRQAKDHCDP KVLCIINPGN PTGQVQSRKC IEDVIHFAWE EKLFLLADEV YQDNVYSPDC RFHSFKKVLY QMGHEYSSNV ELASFHSTSK GYMGECGYRG GYMEVINLHP EIKGQLVKLL SVRLCPPVSG QAAMDIVVNP PEPGEESFEQ FSREKEFVLG NLAKKAKLTE DLFNQVPGIQ CNPLQGAMYA FPRILIPAKA VEAAQSHKMA PDMFYCMKLL EETGICVVPG SGFGQREGTY HFRMTILPPV DKLKTVLHKV KDFHLKFLEQ.

Product Science Overview

Gene and Protein Structure

The GPT2 gene is located on chromosome 8 in mice . The gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that is essential for various metabolic processes . The enzyme is predominantly expressed in tissues such as skeletal muscle, kidney, and liver .

Function and Biological Role

GPT2 is involved in several key biological processes:

  • Amino Acid Metabolism: It facilitates the conversion of alanine to pyruvate, which can then enter the tricarboxylic acid (TCA) cycle for energy production .
  • Gluconeogenesis: By generating pyruvate, GPT2 plays a role in the synthesis of glucose from non-carbohydrate sources, which is vital during fasting or intense exercise .
  • Metabolic Stress Response: The enzyme is upregulated under metabolic stress conditions, particularly in hepatocyte cell lines, indicating its role in maintaining metabolic homeostasis .
Phenotypic Effects

Mice with a knockout allele of the GPT2 gene exhibit several phenotypic abnormalities, including:

  • Hypoactivity: Reduced physical activity levels .
  • Reduced Postnatal Brain Growth: Impaired brain development after birth .
  • Metabolic Defects: Various metabolic issues related to amino acid metabolism and the TCA cycle .
  • Premature Death: Early mortality due to the aforementioned defects .
Research and Applications

Recombinant GPT2 from mice is widely used in research to study its role in metabolism and its potential implications in metabolic disorders. Understanding the function and regulation of GPT2 can provide insights into conditions such as diabetes, obesity, and other metabolic diseases.

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