GPT2 Mouse, Active

Glutamic-Pyruvate Transaminase 2, Active Mouse Recombinant
Cat. No.
BT1822
Source

Escherichia Coli.

Synonyms

ALT2, AAT2, Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2.

Appearance
Sterile Filtered colorless solution.
Purity

Greater than 85.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GPT2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 542 amino acids (1-522 aa) and having a molecular mass of 60.1kDa.
GPT2 is fused to a 20 amino acid His tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Glutamic-Pyruvate Transaminase 2 (GPT2) catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. GPT2 is primarily expressed in muscle, fat, and kidney tissues and plays a role in the intermediary metabolism of glucose and amino acids.
Description
Recombinant GPT2 Mouse is produced in E. coli. It is a single, non-glycosylated polypeptide chain consisting of 542 amino acids (1-522 aa) and has a molecular weight of 60.1 kDa. The protein contains a 20 amino acid His tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The GPT2 solution (1 mg/ml) is supplied in a buffer containing 2mM DTT, 20% Glycerol, and 20mM Tris-HCl (pH 7.5).
Stability
For short-term storage (2-4 weeks), store the solution at 4°C. For extended storage, freeze the solution at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Biological Activity
Specific activity is > 50 units/mg. Activity is determined by measuring the amount of enzyme that catalyzes the conversion of 1 μmole of L-Alanine to L-Glutamate per minute at pH 7.5 and 37°C.
Synonyms

ALT2, AAT2, Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MQRAAVLVRR GSCPRASGPW GRSHSSAAAE ASAALKVRPE
RSPRDRILTL ESMNPQVKAV EYAVRGPIVL KAGEIEMELQ RGIKKPFTEV IRANIGDAHA
MGQQPITFLR QVMALCTYPN LLNSPSFPED AKKRARRILQ ACGGNSLGSY SASQGVNCIR
EDVAAFITRR DGVPADPDNI YLTTGASDGI STILKLLVSG GGKSRTGVMI PIPQYPLYSA
VISELDAVQV NYYLDEENCW ALNVDELRRA LRQAKDHCDP KVLCIINPGN PTGQVQSRKC
IEDVIHFAWE EKLFLLADEV YQDNVYSPDC RFHSFKKVLY QMGHEYSSNV ELASFHSTSK
GYMGECGYRG GYMEVINLHP EIKGQLVKLL SVRLCPPVSG QAAMDIVVNP PEPGEESFEQ
FSREKEFVLG NLAKKAKLTE DLFNQVPGIQ CNPLQGAMYA FPRILIPAKA VEAAQSHKMA
PDMFYCMKLL EETGICVVPG SGFGQREGTY HFRMTILPPV DKLKTVLHKV KDFHLKFLEQ YS

Product Science Overview

Function and Mechanism

GPT2 catalyzes the reversible transamination between alanine and 2-oxoglutarate to produce pyruvate and glutamate . This reaction is essential for the gluconeogenesis and amino acid metabolism in various tissues, including skeletal muscle, kidney, and liver . The enzyme’s activity is dependent on the cofactor pyridoxal phosphate .

Genetic and Molecular Information

The GPT2 gene is located on chromosome 8 in mice . It has multiple transcript variants due to alternative splicing . The gene is upregulated by activating transcription factor 4 (ATF4) under metabolic stress conditions in hepatocyte cell lines .

Biological Significance

GPT2 is involved in several biological processes, including the L-alanine catabolic process, cellular amino acid biosynthetic process, and 2-oxoglutarate metabolic process . It is also associated with the mitochondrial matrix, where it performs its enzymatic functions .

Clinical Relevance

Mutations in the GPT2 gene have been linked to developmental encephalopathy, a condition characterized by impaired brain development . Additionally, GPT2 is used as an indicator of liver function and hepatocellular damage in clinical tests .

Research and Applications

Recombinant GPT2, particularly the mouse variant, is widely used in research to study its role in metabolism and its potential implications in various diseases. The enzyme’s activity and expression patterns provide valuable insights into metabolic pathways and their regulation .

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