GPT2 Human, Active
Glutamic-Pyruvate Transaminase 2 Human Recombinant, Active
Cat. No.
BT1679
Source
E.coli.
Synonyms
ALT2, AAT2, Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
GPT2 Human Recombinant produced in E. coli is a single polypeptide chain containing 546 amino acids (1-523) and having a molecular mass of 60.3 kDa. GPT2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Alanine aminotransferase 2 (GPT2) is an enzyme that facilitates the reversible transamination between alanine and 2-oxoglutarate, resulting in the production of pyruvate and glutamate. GPT2 is primarily found in muscle, fat, and kidney tissues and plays a role in the metabolism of glucose and amino acids. There are multiple transcript variants of GPT2, which encode different isoforms of the enzyme.
Description
Recombinant human GPT2, produced in E. coli, is a single polypeptide chain with a molecular weight of 60.3 kDa. It consists of 546 amino acids, including a 23 amino acid His-tag at the N-terminus (amino acids 1-523). The protein is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The GPT2 solution has a concentration of 0.5 mg/ml and is supplied in a buffer containing 20mM Tris-HCl (pH 7.5), 30% glycerol, 2mM DTT, and 0.2M NaCl.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 90% as determined by SDS-PAGE analysis.
Biological Activity
The specific activity of the enzyme is greater than 100 units/mg. One unit of activity is defined as the amount of enzyme required to catalyze the conversion of 1 μmole of L-Alanine to L-Glutamate per minute at a pH of 7.5 and a temperature of 37°C.
Synonyms
ALT2, AAT2, Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH
SSGLVPRGSH MGSMQRAAAL
VRRGCGPRTP SSWGRSQSSA AAEASAVLKV RPERSRRERI LTLESMNPQV KAVEYAVRGP IVLKAGEIEL
ELQRGIKKPF TEVIRANIGD AQAMGQQPIT FLRQVMALCT YPNLLDSPSF PEDAKKRARR ILQACGGNSL
GSYSASQGVN CIREDVAAYI TRRDGGVPAD PDNIYLTTGA SDGISTILKI LVSGGGKSRT GVMIPIPQYP
LYSAVISELD AIQVNYYLDE ENCWALNVNE LRRAVQEAKD HCDPKVLCII NPGNPTGQVQ SRKCIEDVIH
FAWEEKLFLL ADEVYQDNVY SPDCRFHSFK KVLYEMGPEY SSNVELASFH STSKGYMGEC GYRGGYMEVI
NLHPEIKGQL VKLLSVRLCP PVSGQAAMDI VVNPPVAGEE SFEQFSREKE SVLGNLAKKA KLTEDLFNQV PGIHCNPLQG
AMYAFPRIFI PAKAVEAAQA HQMAPDMFYC MKLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPVEKLKTV
LQKVKDFHIN FLEKYA.
Product Science Overview
Gene and Protein Structure
The GPT2 gene is located on chromosome 16 at the band 16q11.2 . The gene encodes a pyridoxal phosphate-dependent enzyme, which is essential for its catalytic activity . The enzyme is predominantly expressed in tissues such as skeletal muscle, kidney, and liver, where it contributes to various metabolic processes .
Function and Biological Role
GPT2 is involved in several key biological processes, including:
- Amino Acid Metabolism: It facilitates the conversion of alanine to pyruvate, which can then enter the citric acid cycle for energy production .
- Gluconeogenesis: By generating pyruvate, GPT2 supports the synthesis of glucose from non-carbohydrate sources, which is vital during periods of fasting or intense exercise .
- Metabolic Stress Response: The expression of GPT2 is upregulated by activating transcription factor 4 (ATF4) under metabolic stress conditions, particularly in hepatocyte cell lines .
Clinical Significance
Recombinant GPT2
Recombinant human GPT2 is produced using genetic engineering techniques, where the GPT2 gene is inserted into a suitable expression system, such as bacteria or yeast, to produce the active enzyme. This recombinant enzyme is used in various research and diagnostic applications to study its function and role in metabolic processes.
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