GOT1 Mouse

GOT1 is a homodimeric enzyme, meaning it consists of two identical subunits. It catalyzes the reversible transfer of an amino group from glutamate to oxaloacetate, producing aspartate and alpha-ketoglutarate . This reaction is essential for the biosynthesis of L-glutamate from L-aspartate or L-cysteine . GOT1 is also involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis .

GOT1 is an important regulator of glutamate levels, the major excitatory neurotransmitter in the vertebrate central nervous system . It acts as a scavenger of glutamate in brain neuroprotection, helping to maintain the balance of neurotransmitters . Additionally, GOT1’s activity is crucial for the metabolism of amino acids and the production of energy through the tricarboxylic acid cycle .

Recombinant GOT1, particularly from mouse models, is widely used in research to study its function and role in various biological processes. The recombinant form is produced using genetic engineering techniques, where the GOT1 gene is cloned and expressed in a suitable host system, such as bacteria or yeast . This allows for the production of large quantities of the enzyme for experimental purposes.

Alterations in GOT1 activity have been associated with several diseases, including metabolic disorders and certain types of cancer . For instance, KRAS-mutated cancer cells rely on GOT1 to support long-term cell proliferation . Understanding the role of GOT1 in these conditions can provide insights into potential therapeutic targets.