GOT1 Human, Active

GOT1 is a homodimeric enzyme, meaning it consists of two identical subunits. It is involved in the biosynthesis of L-glutamate from L-aspartate or L-cysteine . This process is essential for maintaining the levels of glutamate, which is the major excitatory neurotransmitter in the vertebrate central nervous system . Additionally, GOT1 acts as a scavenger of glutamate in brain neuroprotection .

The enzyme’s activity is also significant in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis . Using L-cysteine as a substrate, GOT1 regulates the levels of mercaptopyruvate, an important source of hydrogen sulfide .

GOT1 is overexpressed in many cancers, making it a potential therapeutic target . Its role in regulating cell proliferation by participating in amino acid metabolism, especially glutamine metabolism, has been highlighted in several studies . This makes GOT1 a critical enzyme in understanding and potentially treating various cancers.

The enzyme is also known as Serum Glutamic-Oxaloacetic Transaminase (SGOT) or Aspartate Aminotransferase (AST). It is commonly measured in blood tests to assess liver health . Elevated levels of SGOT/AST can indicate liver damage or other medical conditions .

Recombinant GOT1 refers to the enzyme produced through recombinant DNA technology, which allows for the production of large quantities of the enzyme for research and therapeutic purposes. This recombinant form retains the same activity and properties as the naturally occurring enzyme, making it valuable for various applications in biochemistry and medicine.