GOT1 Human

GOT1 is involved in the transamination reaction, where it catalyzes the transfer of an amino group from glutamate to oxaloacetate, forming aspartate and alpha-ketoglutarate. This reaction is essential for the urea cycle and the tricarboxylic acid (TCA) cycle, which are critical for energy production and detoxification of ammonia in the body .

GOT1 plays a significant role in the metabolism of amino acids and the regulation of cellular energy. It is particularly important in the biosynthesis of L-glutamate from L-aspartate or L-cysteine, which is a major excitatory neurotransmitter in the central nervous system. Additionally, GOT1 acts as a scavenger of glutamate in brain neuroprotection .

Elevated levels of GOT1 are often associated with liver diseases, such as hepatitis and cirrhosis, as well as myocardial infarction. Measuring the levels of GOT1 in the blood can be used as a diagnostic marker for these conditions .

Human recombinant GOT1 is produced using recombinant DNA technology, which involves inserting the human GOT1 gene into a suitable expression system, such as bacteria or yeast, to produce the enzyme in large quantities. This recombinant enzyme is used in various research and clinical applications, including the study of amino acid metabolism and the development of diagnostic assays .

Recent studies have shown that GOT1 is overexpressed in several types of cancer, making it a potential target for cancer therapy. By inhibiting GOT1, researchers aim to disrupt the metabolic pathways that cancer cells rely on for growth and survival .