GAPDH Human
Glyceraldehyde-3-Phosphate Dehydrogenase Human Recombinant
Cat. No.
BT8219
Source
Escherichia Coli.
Synonyms
G3PD, GAPD, MGC88685, GAPDH, Glyceraldehyde-3-Phosphate Dehydrogenase.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
GAPDH Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 335 amino acids and having a molecular mass of 36kDa.
The GAPDH is purified by proprietary chromatographic techniques.
The GAPDH is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme that plays a crucial role in glycolysis, a metabolic pathway that breaks down glucose to produce energy. GAPDH is a tetrameric enzyme, meaning it consists of four identical subunits, each with a molecular weight of 36 kDa. This enzyme catalyzes the reversible conversion of glyceraldehyde 3-phosphate to 1,3-bisphosphoglycerate, a key step in glycolysis. Beyond its role in energy production, GAPDH is involved in various cellular processes, including membrane fusion, microtubule organization, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair.
Description
Recombinant human GAPDH, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 335 amino acids, resulting in a molecular weight of 36 kDa. The protein is purified using proprietary chromatographic techniques to ensure high purity.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The GAPDH protein is supplied in a solution at a concentration of 1 mg/ml. The buffer consists of 20mM Tris-HCl at pH 8, 1mM EDTA, 1mM DTT, and 20% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. Adding a carrier protein such as 0.1% HSA or BSA is advisable for long-term storage. Repeated freezing and thawing of the protein should be avoided.
Purity
The purity of the GAPDH protein is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
G3PD, GAPD, MGC88685, GAPDH, Glyceraldehyde-3-Phosphate Dehydrogenase.
Source
Escherichia Coli.
Amino Acid Sequence
MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE.
Product Science Overview
Introduction
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a critical enzyme in the glycolytic pathway, catalyzing the sixth step of glycolysis. This enzyme is highly conserved and ubiquitously expressed across various species, including humans. The recombinant form of human GAPDH is often used in research due to its stability and ease of production.
Structure and Localization
GAPDH is a tetrameric protein composed of identical subunits, each with a molecular weight of approximately 37 kDa . The gene encoding GAPDH is located on chromosome 12p13 in humans . While primarily localized in the cytoplasm, GAPDH can translocate to the nucleus under certain cellular conditions .
Biological Functions
Aside from its well-known role in glycolysis, GAPDH has several other functions:
- Energy Metabolism: GAPDH catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, a crucial step in carbohydrate metabolism .
- Protein Interactions: GAPDH binds to various proteins, including actin, tubulin, amyloid precursor, and huntingtin .
- Regulation of Microtubule Dynamics: Phosphorylated GAPDH associates with cytoskeletal elements and controls microtubule dynamics in the early secretory pathway .
- Gene Expression: GAPDH is a component of the functional GAIT (interferon-γ-activated inhibitor of translation) mRNP (messenger ribonucleoprotein) .
Expression Patterns and Tissue Distribution
Regulatory Mechanisms
GAPDH activity is regulated through several mechanisms:
- Post-Translational Modifications: These include phosphorylation, acetylation, and nitrosylation, which can alter the enzyme’s activity and interactions .
- Subcellular Localization: The translocation of GAPDH between the cytoplasm and nucleus is regulated by cellular stress and other signals .
- Protein Oligomerization: The formation of GAPDH oligomers can influence its enzymatic activity and interactions with other proteins .
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