GAPDH Antibody

Glyceraldehyde-3-Phosphate Dehydrogenase, Mouse Anti Human
Cat. No.
BT17786
Source
Synonyms
G3PD, GAPD, MGC88685, GAPDH, Glyceraldehyde-3-Phosphate Dehydrogenase.
Appearance
Sterile Filtered clear solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a crucial enzyme involved in glycolysis, catalyzing the reversible conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. This enzyme, existing as a tetramer of 36-kDa subunits, plays a vital role in energy production within cells. Beyond its glycolytic function in the cytoplasm, GAPDH participates in various cellular processes, including membrane fusion, microtubule organization, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The solution has a concentration of 1mg/ml and contains PBS at pH 7.4 with 0.1% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), keep at 4°C. For long-term storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The product is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This GAPDH antibody has undergone validation through ELISA, Western blot, and Immunofluorescence analyses to confirm its specificity and reactivity. However, optimal working dilutions should be determined empirically for each application. For Western blot and Immunofluorescence, a dilution range of 1:500 to 1:10,000 is recommended, with a starting dilution of 1:1,000.
Synonyms
G3PD, GAPD, MGC88685, GAPDH, Glyceraldehyde-3-Phosphate Dehydrogenase.
Purification Method
GAPDH antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT8G4AT.
Immunogen
Anti-human GAPDH mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human GAPDH amino acids 1-335 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.

Product Science Overview

Introduction

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a crucial enzyme in the glycolytic pathway, catalyzing the sixth step of glycolysis. This enzyme is highly conserved across different species and is ubiquitously expressed in various tissues. The mouse anti-human GAPDH antibody is commonly used in research to detect and quantify GAPDH in human samples.

Structure and Function

GAPDH is a multifunctional protein with a molecular weight of approximately 37 kDa. It is involved in several cellular processes beyond glycolysis, including DNA repair, membrane fusion, and apoptosis . The enzyme’s structure includes a NAD+ binding domain and a catalytic domain, which are essential for its enzymatic activity .

Biological Significance

GAPDH plays a pivotal role in cellular metabolism by facilitating the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate, producing NADH in the process. This reaction is vital for the production of ATP, the energy currency of the cell . Additionally, GAPDH is involved in various non-metabolic processes, such as the regulation of gene expression and the maintenance of cellular homeostasis .

Mouse Anti-Human GAPDH Antibody

The mouse anti-human GAPDH antibody is a monoclonal antibody that specifically binds to human GAPDH. This antibody is widely used in research for Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assays (ELISA). It serves as a loading control in these experiments, ensuring that equal amounts of protein are loaded in each lane or well .

Applications in Research

The mouse anti-human GAPDH antibody is invaluable in various research applications:

  • Western Blotting: Used to confirm the presence and quantity of GAPDH in protein samples.
  • Immunohistochemistry: Helps visualize the localization of GAPDH in tissue sections.
  • ELISA: Quantifies GAPDH levels in different samples, aiding in the study of metabolic and non-metabolic functions of the enzyme .

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