Introduction
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme that plays a crucial role in glycolysis, a metabolic pathway that converts glucose into energy. GAPDH catalyzes the reversible conversion of glyceraldehyde 3-phosphate to 1,3-bisphosphoglycerate. This enzyme is a tetramer, meaning it consists of four identical subunits, each with a molecular weight of 36 kDa. Beyond its function in glycolysis, GAPDH participates in various cellular processes, including membrane fusion, microtubule organization, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair.
Description
This product consists of recombinant mouse GAPDH protein produced in E. coli. It is a single, non-glycosylated polypeptide chain containing 356 amino acids (amino acids 1-333) and has a molecular mass of 38.2 kDa. A 23 amino acid His-tag is fused to the N-terminus of the protein to facilitate purification, which is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The GAPDH protein is provided at a concentration of 0.25 mg/ml in a solution of phosphate-buffered saline (pH 7.4) containing 20% glycerol and 1 mM dithiothreitol (DTT).
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein such as bovine serum albumin (BSA) or human serum albumin (HSA) to a final concentration of 0.1% is advised for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain protein stability.
Purity
The purity of the GAPDH protein is greater than 95% as determined by SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
Synonyms
Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, Gapdh, Gapd, Glyceraldehyde-3-phosphate dehydrogenase isoform 2, G3PD, GAPD, HEL-S-162eP.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMVKVGVN GFGRIGRLVT RAAICSGKVE IVAINDPFID LNYMVYMFQY DSTHGKFNGT VKAENGKLVI NGKPITIFQE RDPTNIKWGE AGAEYVVEST GVFTTMEKAG AHLKGGAKRV IISAPSADAP MFVMGVNHEK YDNSLKIVSN ASCTTNCLAP LAKVIHDNFG IVEGLMTTVH AITATQKTVD GPSGKLWRDG RGAAQNIIPA STGAAKAVGK VIPELNGKLT GMAFRVPTPN VSVVDLTCRL EKPAKYDDIK KVVKQASEGP LKGILGYTED QVVSCDFNSN SHSSTFDAGA GIALNDNFVK LISWYDNEYG YSNRVVDLMA YMASKE.