FOLR2 Human
HEK293 Cells.
Folate receptor beta, FR-beta, Folate receptor 2, Folate receptor, fetal/placental, Placental folate-binding protein, BETA-HFR Protein, FBP/PL-1 Protein, FR-BETA Protein, FR-P3 Protein, FRbeta, FR-BETA, FBP/PL1, BETA-HFR, FBP, FOLR2
Greater than 90.0% as determined by SDS-PAGE.
Description
FOLR2 Human Recombinant is a single, glycosylated polypeptide chain containing 215 amino acids (22-230a.a) and having a molecular mass of 25.1kDa. FOLR2 is fused to a 6 a.a His tag at C-terminal, and is purified by proprietary chromatographic techniques.
Product Specs
The folate receptor family, which includes FOLR1 (Folate Receptor 1), is known for its members' ability to bind folic acid. FOLR1 plays a crucial role in binding folate and its reduced derivatives, facilitating the cellular uptake of 5-methyltetrahydrofolate and folate analogs. This receptor exists in two forms: membrane-bound, anchored by a glycosyl-phosphatidylinositol linkage, and a soluble form. FOLR1 is essential for embryonic development and the regulation of normal cell proliferation.
Recombinant Human FOLR2 is a single, glycosylated polypeptide chain composed of 215 amino acids (22-230a.a), resulting in a molecular weight of 25.1 kDa. A 6 a.a His tag is fused to the C-terminus of FOLR2. Purification is achieved through proprietary chromatographic methods.
The FOLR2 solution is provided at a concentration of 0.25 mg/ml in a buffer containing 10% glycerol and Phosphate-Buffered Saline (pH 7.4).
For short-term storage (2-4 weeks), the entire vial can be stored at 4°C. For extended storage, freezing at -20°C is recommended. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. It is crucial to avoid repeated freeze-thaw cycles to maintain protein integrity.
The purity of this protein is determined to be greater than 90.0% using SDS-PAGE analysis.
The ED50 range for this protein is approximately 5 ug/ml. Biological activity is assessed through a functional ELISA, specifically measuring the protein's binding affinity to Folic Acid-BSA.
Folate receptor beta, FR-beta, Folate receptor 2, Folate receptor, fetal/placental, Placental folate-binding protein, BETA-HFR Protein, FBP/PL-1 Protein, FR-BETA Protein, FR-P3 Protein, FRbeta, FR-BETA, FBP/PL1, BETA-HFR, FBP, FOLR2
HEK293 Cells.
QDRTDLLNVC MDAKHHKTKP GPEDKLHDQC SPWKKNACCT ASTSQELHKD TSRLYNFNWD HCGKMEPACK RHFIQDTCLY ECSPNLGPWI QQVNQSWRKE RFLDVPLCKE DCQRWWEDCH TSHTCKSNWH RGWDWTSGVN KCPAGALCRT FESYFPTPAA LCEGLWSHSY KVSNYSRGSG RCIQMWFDSA QGNPNEEVAR FYAAAMHVNH HHHHH
Product Science Overview
Folate Receptor 2 (FOLR2), also known as folate receptor beta (FRβ), is a member of the folate receptor family. These receptors have a high affinity for folic acid and several reduced folic acid derivatives. FOLR2 plays a crucial role in mediating the delivery of 5-methyltetrahydrofolate to the interior of cells through a process known as potocytosis .
The human FOLR2 protein consists of 223 amino acids and has a predicted molecular mass of approximately 26 kDa. Due to glycosylation, its apparent molecular mass in SDS-PAGE under reducing conditions is around 30-35 kDa . The recombinant form of FOLR2 is typically expressed in HEK293 cells and is often tagged with a polyhistidine tag at the C-terminus for purification purposes .
FOLR2 is predominantly expressed in the placenta, spleen, bone marrow, and thymus . It is also a marker for macrophages generated in the presence of macrophage colony-stimulating factor (M-CSF), but not granulocyte-macrophage colony-stimulating factor (GM-CSF) . The expression of FOLR2 is increased in certain malignant tissues, including myeloid leukemias and head and neck squamous cell carcinomas .
Folate receptors, including FOLR2, are essential for the cellular uptake of folate, a vital nutrient required for DNA synthesis, repair, and methylation. FOLR2 binds to folate and its derivatives with high affinity, facilitating their transport into cells. This process is critical for rapidly dividing cells, such as those in the bone marrow and developing fetus .
The expression of FOLR2 is upregulated in various cancers, making it a potential target for cancer diagnostics and therapeutics. For instance, FOLR2-targeted therapies could be used to deliver cytotoxic agents specifically to cancer cells, minimizing damage to healthy tissues . Additionally, FOLR2’s role in folate uptake makes it a potential biomarker for certain types of cancer and inflammatory diseases .
Recombinant human FOLR2 is produced using DNA sequences encoding the human FOLR2 protein, typically expressed in mammalian cell lines like HEK293. The recombinant protein is purified and often used in research to study folate receptor function, develop targeted therapies, and investigate the role of folate receptors in various diseases .
