FOLR1 Human

Folate Receptor 1 Human Recombinant
Cat. No.
BT9184
Source
Escherichia Coli.
Synonyms
Folate Receptor 1 (Adult), Ovarian Tumor-Associated Antigen MOv18, Adult Folate-Binding Protein, Folate Receptor Adult , KB Cells FBP, FR-Alpha, FOLR, FBP, Folate Binding Protein, Folate Receptor Alpha, Folate Receptor 1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

FOLR1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 233 amino acids (25-234 a.a) and having a molecular mass of 27kDa. FOLR1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The Folate Receptor 1, also referred to as FOLR1, belongs to the folate receptor family, whose members exhibit binding affinity for folic acid. FOLR1 demonstrates the ability to bind folate and its reduced derivatives, facilitating the cellular uptake of 5-methyltetrahydrofolate and folate analogs. FOLR1 exhibits two distinct forms: membrane-bound, anchored via a glycosyl-phosphatidylinositol linkage, and a soluble form. FOLR1 plays a crucial role in embryonic development and the regulation of normal cell proliferation.
Description
Recombinantly produced in E. coli, FOLR1 Human Recombinant is a single, non-glycosylated polypeptide chain comprising 233 amino acids (specifically, residues 25-234). This protein has a molecular weight of 27 kDa. A 23-amino acid His-tag is fused to the N-terminus of FOLR1. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
The product is presented as a sterile, filtered solution with a clear appearance.
Formulation
The FOLR1 protein solution is provided at a concentration of 0.5 mg/ml. The solution is buffered with 20 mM Tris-HCl at a pH of 8.0 and supplemented with 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
Analysis by SDS-PAGE confirms a purity exceeding 90.0%.
Synonyms
Folate Receptor 1 (Adult), Ovarian Tumor-Associated Antigen MOv18, Adult Folate-Binding Protein, Folate Receptor Adult , KB Cells FBP, FR-Alpha, FOLR, FBP, Folate Binding Protein, Folate Receptor Alpha, Folate Receptor 1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSRIAWART ELLNVCMNAK HHKEKPGPED KLHEQCRPWR KNACCSTNTS QEAHKDVSYL YRFNWNHCGE MAPACKRHFI QDTCLYECSP NLGPWIQQVD QSWRKERVLN VPLCKEDCEQ WWEDCRTSYT CKSNWHKGWN WTSGFNKCAV GAACQPFHFY FPTPTVLCNE IWTHSYKVSN YSRGSGRCIQ MWFDPAQGNP NEEVARFYAA AMS.

Product Science Overview

Introduction

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha (FRα) or Folate Binding Protein (FBP), is a high-affinity receptor for folic acid and its reduced derivatives. It plays a crucial role in the cellular uptake of folates, which are essential for various metabolic processes, including nucleotide synthesis, methionine synthesis, and the interconversion of glycine and serine .

Structure and Function

FOLR1 is a glycoprotein that can exist in two forms: membrane-bound and soluble. The membrane-bound form is anchored to the cell surface via a glycosyl-phosphatidylinositol (GPI) linkage, while the soluble form can be found in bodily fluids such as serum and breast milk . The receptor is predominantly expressed on epithelial cells and is significantly upregulated in various carcinomas, including breast and ovarian cancers .

Biological Importance

FOLR1 is essential for embryonic development and normal cell proliferation. It facilitates the transfer of 5-methyltetrahydrofolate and other folate analogs into cells, supporting critical metabolic pathways . Mutations in the FOLR1 gene have been associated with neurodegenerative conditions due to cerebral folate transport deficiency .

Recombinant FOLR1

Recombinant human FOLR1 is produced using various expression systems, including HEK293 and CHO cells. The recombinant protein is typically tagged with a polyhistidine (His) tag to facilitate purification and detection . The recombinant form retains the functional properties of the native protein, making it valuable for research and therapeutic applications.

Applications

Recombinant FOLR1 is used in various research applications, including:

  • Cancer Research: Studying the overexpression of FOLR1 in cancer cells and developing targeted therapies.
  • Drug Delivery: Designing folate-conjugated drugs for targeted delivery to cells expressing FOLR1.
  • Diagnostic Tools: Developing assays to measure FOLR1 levels in biological samples for diagnostic purposes.

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