Flt3-Ligand Rhesus Macaque

The recombinant Flt3-Ligand for rhesus macaques is produced using E. coli expression systems. This recombinant protein is a single, non-glycosylated polypeptide chain containing 159 amino acids and has a molecular mass of approximately 18.0 kDa . The recombinant protein is purified using proprietary chromatographic techniques to ensure high purity and activity .

The biological activity of the recombinant Flt3-Ligand is measured by its ability to stimulate the proliferation of human AML5 cells. The effective dose (ED50) for this activity is less than 1.0 ng/ml, corresponding to a specific activity of 1,000,000 IU/mg . This high level of activity makes it a valuable tool for research in hematopoiesis and immunology.

Flt3-Ligand has been extensively studied for its role in enhancing the immunogenicity of vaccines. For instance, administration of Flt3-Ligand has been shown to promote the expansion of dendritic cell populations in the spleen and blood, which are critical for initiating immune responses . In rhesus macaques, the use of a rhesus-specific engineered Flt3L-Fc fusion protein has demonstrated significant enhancement in vaccine-induced T-cell responses .

The lyophilized form of recombinant Flt3-Ligand is stable at room temperature for up to three weeks. However, for long-term storage, it should be kept desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C for short-term use (2-7 days) and below -18°C for future use . It is important to avoid repeated freeze-thaw cycles to maintain the protein’s stability and activity .