Flt3 Ligand Human, Sf9
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Flt3-Ligand is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Flt3 Ligand (Fms-like tyrosine kinase 3 ligand) is a crucial growth factor involved in the regulation of hematopoiesis, the process by which blood cells are formed. This ligand binds to the Flt3 receptor, which is expressed on various hematopoietic progenitor cells, including myeloid, lymphoid, and dendritic cell progenitors .
Recombinant Human Flt3 Ligand is produced using the baculovirus expression system in Sf9 insect cells. This method allows for the production of a glycosylated polypeptide chain containing 155 amino acids, which migrates on SDS-PAGE at approximately 19 kDa . The recombinant protein is purified using proprietary chromatographic techniques to ensure high purity and biological activity .
Flt3 Ligand plays a significant role in the proliferation and differentiation of hematopoietic stem cells. It works synergistically with other growth factors such as granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), macrophage colony-stimulating factor (M-CSF), interleukin-3 (IL-3), and stem cell factor (SCF) to promote the growth and differentiation of primitive hematopoietic cells . Additionally, Flt3 Ligand is crucial for the differentiation of B cells and natural killer (NK) cells in the lymphoid lineage and dendritic cells in the myeloid lineage .
Recombinant Human Flt3 Ligand is widely used in research for various applications, including:
- In vitro expansion of CD34+ hematopoietic progenitor cells: This application is essential for studying hematopoiesis and developing potential therapeutic strategies for blood-related disorders .
- Differentiation studies: Flt3 Ligand is used to differentiate embryonic stem (ES) cells towards the hematopoietic lineage, providing insights into early blood cell development .
- Generation of dendritic cells and other immune cells: The ligand is used to generate Langerhans cells, dendritic cells, and eosinophils from CD34+ cells, which are crucial for immune response studies .
The lyophilized recombinant Human Flt3 Ligand is stable at room temperature for up to three weeks. However, for long-term storage, it should be kept desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term use. It is recommended to add a carrier protein, such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA), to prevent freeze-thaw cycles .
