Flt3 Ligand Mouse, Sf9
Description
Flt3 Ligand shows multiple bands between 18-28kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Flt3 Ligand is produced as a recombinant protein in various systems, including Sf9 insect cells. The recombinant form is optimized for use in cell culture, differentiation studies, and functional assays. The protein is typically expressed as a soluble isoform, although it can also exist as a membrane-bound form. The recombinant mouse Flt3 Ligand produced in Sf9 cells is characterized by high purity and biological activity, making it suitable for research applications .
Flt3 Ligand functions by binding to its receptor, Flt3 (also known as Flk-2), which is a tyrosine kinase receptor expressed on hematopoietic progenitor cells. This interaction stimulates the proliferation and differentiation of primitive hematopoietic stem cells. Flt3 Ligand works synergistically with other growth factors such as G-CSF, GM-CSF, SCF, and IL-3 to promote early B cell lineage differentiation and the expansion of monocytes and immature dendritic cells .
Recombinant mouse Flt3 Ligand has several applications in biomedical research:
- Dendritic Cell Generation: It is used to induce the maturation and expansion of dendritic cells (DCs) in vivo and for the in vitro generation of bone marrow-derived DCs.
- Eosinophil Generation: It aids in the generation of eosinophils from bone marrow progenitor cells.
- Allergic Inflammation Studies: It is utilized in studies on allergic airway inflammation in mouse models.
- Hematopoietic Stem Cell Research: It is employed in studies focusing on the proliferation and differentiation of hematopoietic stem cells .
The recombinant mouse Flt3 Ligand produced in Sf9 cells is of research-grade quality. It is sterile-filtered prior to lyophilization, with endotoxin levels typically below 0.1 ng/μg and purities exceeding 95%. The biological activity of the protein is tested in appropriate bioassays to ensure its efficacy in research applications .
