Flt3 Ligand Mouse
Greater than 95.0% as determined by SDS-PAGE.
Description
Flt3-Ligand Mouse Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containing 163 amino acids and having a molecular mass of 18.6kDa. Flt3-Ligand is purified by proprietary chromatographic techniques.
Product Specs
FLT3 ligand, a receptor for the fl cytokine, possesses tyrosine-protein kinase activity and functions as a growth factor. It plays a crucial role in regulating the proliferation of early-stage hematopoietic cells. Moreover, Flt3-Ligand exhibits synergistic effects with other CSFs and interleukins, thereby inducing both growth and differentiation processes.
Recombinant Flt3-Ligand Mouse, expressed in E. coli, is a non-glycosylated polypeptide chain. It comprises 163 amino acids, resulting in a molecular weight of 18.6 kDa. The purification of Flt3-Ligand is achieved through proprietary chromatographic methods.
The product appears as a sterile, white powder that has been lyophilized (freeze-dried).
The lyophilization of FLT3L Mouse protein was performed using a sterile filtered solution containing 10 mM sodium phosphate at a pH of 7.5.
To reconstitute the lyophilized Flt3l Mouse recombinant, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of 100 μg/ml. This solution can be further diluted as needed in other aqueous solutions.
Lyophilized Flt3-Ligand demonstrates stability at room temperature for a period of 3 weeks. However, it is advisable to store it in a desiccated state below -18°C. Once reconstituted, Flt-3 Ligand should be stored at 4°C for 2-7 days. For extended storage, it is recommended to freeze it below -18°C. To ensure optimal long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. It is crucial to avoid repeated freeze-thaw cycles.
The purity of the product is determined to be greater than 95.0% based on SDS-PAGE analysis.
The ED50 value, representing the effective dose at which 50% of mouse AML5 cells proliferate, was calculated to be 4.96 ng/ml. This corresponds to a specific activity of 2.0 x 105 units/mg.
Product Science Overview
Flt3 Ligand is a small molecule that acts by binding to the Flt3 receptor (Fms-like tyrosine kinase 3), which is expressed on the surface of hematopoietic progenitor cells. This binding activates the receptor, leading to a cascade of intracellular signaling pathways that promote cell growth and differentiation .
In mice, the recombinant form of Flt3 Ligand is produced using various expression systems, including E. coli and baculovirus-infected insect cells. The recombinant protein is typically purified to high levels of purity, often exceeding 97%, and is used in various research applications .
Flt3 Ligand is known for its ability to increase the number of immune cells by activating hematopoietic progenitors. It is particularly important for the development of dendritic cells, which are key players in the immune system. The ligand also induces the mobilization of hematopoietic progenitors and stem cells in vivo .
The Flt3 Ligand has potential therapeutic applications, particularly in the context of bone marrow transplantation and cancer treatment. By promoting the proliferation and differentiation of hematopoietic progenitor cells, Flt3 Ligand can enhance the recovery of the immune system following chemotherapy or radiation therapy .
