FGFR3 Human

Fibroblast Growth Factor Receptor 3 Fc Chimera Human Recombinant
Cat. No.
BT19772
Source
Insect Cells.
Synonyms
Achondroplasia, Thanatophoric Dwarfism, CD333, ACH, CEK2, JTK4, HSFGFR3EX, FGFR3.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90.0% as determined by:
(a) Analysis by silver stain.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Soluble FGFR3 Human recombinant fused using a Xa cleavage site with the Fc part of human IgG1 produced in Fc Chimera is a heterodimeric, glycosylated, Polypeptide chain containing 593aa and having a molecular mass of 170kDa. 

Product Specs

Introduction
The Fibroblast Growth Factor (FGF) family consists of at least 18 structurally related proteins, playing diverse roles in physiological and pathological processes such as cell growth, differentiation, angiogenesis, wound healing, and tumorigenesis. FGFs exert their biological activities by binding to and activating a family of type I transmembrane tyrosine kinase receptors known as FGFRs. Upon ligand binding, FGFRs dimerize and undergo autophosphorylation, initiating downstream signaling cascades. Currently, four distinct genes encoding highly similar FGF receptors, FGFR-1 to -4, have been identified. Alternative splicing of mRNAs gives rise to multiple isoforms of FGFR-1 to -3. A common splicing event affects FGFR-1 and -2, resulting in receptors that either contain all three immunoglobulin-like domains (IgI, IgII, and IgIII), referred to as the alpha isoform, or only IgII and IgIII, termed the beta isoform. For FGFR-3 and FGFR-4, only the alpha isoform has been observed. Further splicing events in FGFR-1 to -3, specifically involving the C-terminal region of the IgIII domain encoded by two mutually exclusive exons, generate FGF receptors with variant IgIII domains (IIIb and IIIc). Additionally, a IIIa isoform of FGFR-1 has been reported, which acts as a secreted FGF binding protein and comprises the N-terminal half of the IgIII domain along with some intronic sequences. Notably, mutations in FGFR-1 to -3 have been linked to birth defects characterized by craniosynostosis.
Description
Soluble FGFR3 Human recombinant, a heterodimeric, glycosylated polypeptide chain containing 593 amino acids with a molecular weight of 170 kDa, is produced in Fc Chimera by fusing the protein with the Fc portion of human IgG1 using a Xa cleavage site.
Physical Appearance
The product appears as a sterile, white, lyophilized (freeze-dried) powder.
Formulation
FGFR3 is lyophilized from a sterile solution at a concentration of 1 mg/ml in phosphate-buffered saline (PBS).
Solubility
To reconstitute the lyophilized FGFR-3, it is recommended to dissolve it in sterile PBS at a concentration of at least 50 µg/ml. The reconstituted solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized FGFR3, while stable at room temperature for up to 3 weeks, should be stored desiccated at a temperature below -18°C. After reconstitution, FGFR3 should be stored at 4°C for 2-7 days. For long-term storage, it is recommended to freeze the reconstituted FGFR3 below -18°C after adding a carrier protein, such as 0.1% HSA or BSA. Avoid repeated freeze-thaw cycles to maintain protein stability.
Purity
The purity of FGFR3 is greater than 90%, as determined by: (a) Silver stain analysis and (b) SDS-PAGE analysis.
Synonyms
Achondroplasia, Thanatophoric Dwarfism, CD333, ACH, CEK2, JTK4, HSFGFR3EX, FGFR3.
Source
Insect Cells.
Amino Acid Sequence
ESLGTEQRVV GRAAEVPGPE PGQQEQLVFG SGDAVELSCP PPGGGPMGPT VWVKDGTGLV PSERVLVGPQ RLQVLNASHE DSGAYSCRQR LTQRVLCHFS VRVTDAPSSG DDEDGEDEAE DTGVDTGAPY WTRPERMDKK LLAVPAANTV RFRCPAAGNP TPSISWLKNG REFRGEHRIG GIKLRHQQWS LVMESVVPSD RGNYTCVVEN KFGSIRQTYT LDVLERSPHR PILQAGLPAN QTAVLGSDVE FHCKVYSDAQ PHIQWLKHVE VNGSKVGPDG TPYVTVLKTA GANTTDKELE VLSLHNVTFE DAGEYTCLAG NSIGFSHHSA WLVVLPAEEE LVEADEAGDP RRASIEGRGD PEEPKSCDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK. 

Product Science Overview

Introduction

Fibroblast Growth Factor Receptor 3 (FGFR3) is a member of the fibroblast growth factor receptor family, which plays a crucial role in various cellular processes including cell growth, differentiation, angiogenesis, wound healing, and tumorigenesis . FGFR3 is a transmembrane tyrosine kinase receptor that belongs to the immunoglobulin (Ig) superfamily and binds to fibroblast growth factors (FGFs) . The receptor is involved in cell survival, proliferation, migration, and differentiation .

Structure and Isoforms

FGFR3 consists of an extracellular ligand-binding domain, a single transmembrane domain, and an intracellular tyrosine kinase domain . The extracellular domain contains three immunoglobulin-like (Ig-like) loops, a short cluster of conserved acidic amino acids, and a heparin-binding domain . Alternative splicing in the Ig-like loop III results in IIIb and IIIc isoforms of FGFR1-3, which change the receptor specificity . FGFR3 IIIc binds many FGFs including FGF-1, -2, -4, -6, -8, -9, -18, -19, and -20, while FGFR IIIb only binds to FGF-1, -9, and -16 .

FGFR3 Fc Chimera

The FGFR3 Fc Chimera is a recombinant protein that combines the extracellular domain of FGFR3 with the Fc region of human IgG1 . This fusion protein is designed to enhance the stability and solubility of FGFR3, making it suitable for various research applications . The Fc region also allows for easy purification and detection using Fc-specific antibodies .

Preparation and Production

The recombinant FGFR3 Fc Chimera is typically produced in mammalian cell lines such as NS0 or 293E cells . The protein is expressed with a linker sequence and a C-terminal human IgG1, resulting in a disulfide-linked homodimer . The recombinant protein is then purified using affinity chromatography and characterized by SDS-PAGE to ensure high purity and correct molecular weight .

Applications and Significance

FGFR3 Fc Chimera is widely used in research to study the role of FGFR3 in various cellular processes and diseases . It is particularly useful in cancer research, as FGFR3 is one of the most mutated genes in human cancers . The recombinant protein can be used to inhibit FGF-dependent proliferation of cells, making it a valuable tool for studying the signaling pathways involved in cancer and other diseases .

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