FGFR3 Human, His

The fibroblast growth factor (FGF) family consists of at least 18 structurally related proteins with diverse roles in physiological and pathological processes, such as cell growth, differentiation, angiogenesis, wound healing, and tumorigenesis. FGFs exert their biological effects by binding to and activating a family of type I transmembrane tyrosine kinase receptors known as fibroblast growth factor receptors (FGFRs). Upon ligand binding, FGFRs undergo dimerization and autophosphorylation. Four distinct genes (FGFR-1 to -4) encode these closely related receptors. Alternative splicing of mRNAs gives rise to multiple isoforms of FGFR-1 to -3. A common splicing event in FGFR-1 and -2 produces receptors containing all three immunoglobulin-like domains (Ig domains), known as the alpha isoform, or only IgII and IgIII, known as the beta isoform. Notably, only the alpha isoform has been identified for FGFR-3 and FGFR-4. Further splicing events in FGFR-1 to -3, specifically in the C-terminal half of the IgIII domain encoded by two mutually exclusive exons, generate receptors with alternative IgIII domains (IIIb and IIIc). Additionally, a secreted FGF-binding protein called the IIIa isoform has been reported for FGFR-1. This isoform comprises the N-terminal half of the IgIII domain and some intron sequences. Mutations in FGFR-1 to -3 have been implicated in birth defects involving craniosynostosis.

Recombinant human FGFR3, produced in Sf9 insect cells using a baculovirus expression system, is a single glycosylated polypeptide chain. This protein consists of 592 amino acids (residues 23-375a.a.) and has a molecular weight of 65.1 kDa. Note that its apparent molecular size on SDS-PAGE may appear between 70-100 kDa.

This FGFR3 protein is expressed with a C-terminal 239 amino acid hIgG-His tag and purified using proprietary chromatographic methods.

The FGFR3 protein solution is provided at a concentration of 1 mg/ml and is formulated in phosphate-buffered saline (PBS) at pH 7.4 with 10% glycerol.

For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability during frozen storage, consider adding a carrier protein (0.1% HSA or BSA). It's important to avoid repeated freeze-thaw cycles to maintain protein integrity.

The purity of this protein is determined to be greater than 95.0% as assessed by SDS-PAGE analysis.

CD333 Antigen, HSFGFR3EX, EC 2.7.10, CD333, CEK2, ACH, Fibroblast Growth Factor Receptor 3, EC 2.7.10.1, FGFR-3, JTK4, Fibroblast Growth Factor Receptor 3 Variant 4, Achondroplasia, Thanatophoric Dwarfism, Hydroxyaryl-Protein Kinase, Tyrosine Kinase JTK4.

ESLGTEQRVV GRAAEVPGPE PGQQEQLVFG SGDAVELSCP PPGGGPMGPT VWVKDGTGLV PSERVLVGPQ RLQVLNASHE DSGAYSCRQR LTQRVLCHFS VRVTDAPSSG DDEDGEDEAE DTGVDTGAPY WTRPERMDKK LLAVPAANTV RFRCPAAGNP TPSISWLKNG REFRGEHRIG GIKLRHQQWS LVMESVVPSD RGNYTCVVEN KFGSIRQTYT LDVLERSPHR PILQAGLPAN QTAVLGSDVE FHCKVYSDAQ PHIQWLKHVE VNGSKVGPDG TPYVTVLKTA GANTTDKELE VLSLHNVTFE DAGEYTCLAG NSIGFSHHSA WLVVLPAEEE LVEADEAGSV YAGLEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH.