FGF 2 Antibody

Fibroblast Growth Factor-2, Mouse Anti-Human
Cat. No.
BT5036
Source
Synonyms
Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.
Appearance
Purity
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Basic fibroblast growth factor (FGF2) belongs to the fibroblast growth factor (FGF) family. FGF family members exhibit a wide range of mitogenic and cell survival activities and play crucial roles in various biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, and tumor growth and invasion. FGF2 functions as a regulator of endothelial cell migration and proliferation and acts as an angiogenic factor. It stimulates the proliferation of diverse mesoderm- and neuroectoderm-derived cells in vitro, suggesting its involvement in organogenesis. Three alternatively spliced variants encoding different FGF2 isoforms have been identified. Heparin-binding growth factors, including FGF2, exhibit angiogenic properties in vivo and act as potent mitogens for various cell types in vitro. Notably, there are variations in the tissue distribution and concentration of these growth factors.
Formulation
The antibody is supplied as a 1 mg/ml solution in phosphate-buffered saline (PBS) after reconstitution.
Shipping Conditions
The antibody is shipped in lyophilized form at ambient temperature.
Storage Procedures
For long-term storage, keep the lyophilized antibody at 4°C in a dry environment. After reconstitution, aliquot the antibody and store at -20°C if not intended for use within one month.
Solubility
To reconstitute the antibody, add sterile water (H2O) to the vial. Mix gently by swirling, ensuring the solution reaches the sides of the vial, and wait for 30-60 seconds before use.
Applications
The antibody is suitable for use in various applications, including direct enzyme-linked immunosorbent assay (ELISA), Western blotting, and immunoprecipitation.
Titer
In a direct ELISA, a 1:10,000 dilution of the antibody yields an optical density (O.D.) of 0.8 when using alkaline phosphatase-conjugated rabbit anti-mouse immunoglobulin G (IgG) secondary antibody (Jackson Laboratories).
Synonyms
Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.
Purification Method
Ion exchange.
Type
Mouse Anti Human Monoclonal.
Clone
NYR-hFGF-b.
Immunogen
r.Human FGF-b.
Ig Subclass
Mouse IgM.

Product Science Overview

Introduction

Fibroblast Growth Factor-2 (FGF-2), also known as basic fibroblast growth factor (bFGF), is a member of the fibroblast growth factor family. This family consists of proteins involved in various biological processes, including cell growth, development, and tissue repair. FGF-2 is particularly notable for its role in angiogenesis, wound healing, and embryonic development.

Structure and Function

FGF-2 is a heparin-binding protein that exists in multiple isoforms due to alternative translation initiation sites. These isoforms vary in their molecular weight and cellular localization. The low molecular weight (18 kDa) isoform is predominantly found in the cytoplasm and extracellular matrix, while the high molecular weight isoforms (22-34 kDa) are primarily nuclear.

FGF-2 exerts its biological effects by binding to specific cell surface receptors known as fibroblast growth factor receptors (FGFRs). This binding triggers a cascade of downstream signaling pathways, including the MAPK/ERK, PI3K/Akt, and PLCγ pathways, which ultimately lead to cellular proliferation, differentiation, and survival.

Role in Development and Disease

FGF-2 plays a crucial role in various physiological and pathological processes:

  • Angiogenesis: FGF-2 is a potent angiogenic factor that promotes the formation of new blood vessels from pre-existing ones. This process is essential for wound healing and tissue regeneration.
  • Wound Healing: FGF-2 accelerates wound healing by stimulating the proliferation and migration of fibroblasts and endothelial cells.
  • Embryonic Development: FGF-2 is involved in the development of various tissues and organs during embryogenesis, including the nervous system, skeletal system, and cardiovascular system.
  • Cancer: Aberrant expression of FGF-2 has been implicated in tumorigenesis. FGF-2 promotes cancer cell proliferation, invasion, and metastasis by enhancing angiogenesis and modulating the tumor microenvironment .
Mouse Anti-Human FGF-2 Antibody

Mouse anti-human FGF-2 antibodies are monoclonal antibodies produced by immunizing mice with human FGF-2. These antibodies are highly specific and are used in various research and diagnostic applications, including:

  • Western Blotting: Detecting FGF-2 protein levels in cell and tissue lysates.
  • Immunohistochemistry: Localizing FGF-2 in tissue sections.
  • ELISA: Quantifying FGF-2 levels in biological samples.
  • Flow Cytometry: Analyzing FGF-2 expression on the cell surface.

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