FGF 2 Human
Prostatropin, FGF-basic, Basic FGF, HBGF-2, FGF-2, FGF-b.
Greater than 98.0% as determined by Analysis by SDS-PAGE.
Description
The FGF-b is purified by proprietary chromatographic techniques.
Product Specs
Fibroblast growth factor-2 (FGF-2), also known as basic fibroblast growth factor (bFGF), is a member of the fibroblast growth factor (FGF) family. FGFs are multifunctional proteins with a wide range of biological activities, including roles in development, tissue repair, and disease. FGF-2 is a potent mitogen for a variety of cell types, including fibroblasts, endothelial cells, and epithelial cells. It plays an important role in angiogenesis (blood vessel formation), wound healing, and embryonic development.
The protein was lyophilized from a concentrated (1 mg/mL) solution in 20 mM Tris-HCl, pH 7.4, and 1 M NaCl.
Greater than 98.0% as determined by SDS-PAGE analysis.
The ED50, as determined by the dose-dependent proliferation of murine balb/c 3T3 cells, is less than 0.1 ng/mL, corresponding to a specific activity greater than 1.0 x 107 Units/mg.
Prostatropin, FGF-basic, Basic FGF, HBGF-2, FGF-2, FGF-b.
Product Science Overview
FGF-basic is a non-glycosylated, heparin-binding growth factor. The human recombinant form of FGF-basic is a protein consisting of 154 amino acid residues, with a molecular weight of approximately 17.2 kDa . The amino acid sequence of FGF-basic is as follows:
AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK
LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT
SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS
FGF-basic is known for its ability to stimulate the proliferation and differentiation of a wide variety of cells, including mesenchymal, neuroectodermal, and endothelial cells . It exerts potent angiogenic activity, promoting the formation of new blood vessels, which is essential for wound healing and tissue repair .
Recombinant human FGF-basic is widely used in research and clinical applications. It is particularly valuable in stem cell research, where it is used to promote the proliferation and differentiation of stem cells . Additionally, FGF-basic is used in studies related to angiogenesis, wound healing, and tissue regeneration .
The recombinant form of FGF-basic is typically purified to a high degree, with a purity of ≥ 95% as determined by SDS-PAGE gel and HPLC analyses . Its biological activity is assessed using cell proliferation assays, with an expected ED50 of ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10^7 units/mg .
