FGF 2 Rat
Fibroblast Growth Factor-basic Rat Recombinant
Cat. No.
BT6819
Source
Escherichia Coli.
Synonyms
HBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b, Fibroblast Growth Factor-basic, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished form LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
bFGF Rat Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16.4 kDa.
The bFGF2 is purified by proprietary chromatographic techniques.
The bFGF2 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Fibroblast growth factor-basic (FGF-basic), also known as FGF-2, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and angiogenic activities and bind heparin. FGF-basic is involved in various biological processes, including wound healing, limb and nervous system development, and tumor growth. The mRNA for this gene undergoes alternative translation from AUG and non-AUG (CUG) initiation codons and contains multiple polyadenylation sites, resulting in five different isoforms with distinct properties. The AUG-initiated form is primarily cytosolic and is responsible for the autocrine and paracrine effects of FGF-basic, whereas the CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect. Heparin-binding growth factors, including FGF-basic, exhibit potent mitogenic activity for various cell types in vitro and act as angiogenic agents in vivo. Notably, there are differences in the tissue distribution and concentration of these growth factors.
Description
Recombinant Rat FGF-basic (FGF-2) is a single, non-glycosylated polypeptide chain produced in E. coli. It comprises 146 amino acids, has a molecular weight of 16.4 kDa, and is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
FGF-basic was lyophilized from a 1 mg/ml solution in 10 mM Na₂PO₄, pH 7.5, and 50 mM NaCl after extensive dialysis.
Solubility
Reconstitute the lyophilized Rat bFGF in sterile 18 MΩ-cm H₂O to a concentration of at least 100 µg/ml. The reconstituted solution can be further diluted in other aqueous solutions.
Stability
Lyophilized Rat bFGF remains stable at room temperature for 3 weeks. However, it is recommended to store the lyophilized powder desiccated at temperatures below -18°C. Upon reconstitution, Rat FGF-2 should be stored at 4°C for 2-7 days or at temperatures below -18°C for future use. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Greater than 95.0% purity as determined by SDS-PAGE analysis.
Biological Activity
The ED₅₀ range, determined by the dose-dependent proliferation of NR6R-3T3 cells, was found to be 0.903 ng/ml. This corresponds to a specific activity of 1.1 × 10⁷ units/mg.
Synonyms
HBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b, Fibroblast Growth Factor-basic, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2.
Source
Escherichia Coli.
Amino Acid Sequence
MPALPEDGGGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS.
Product Science Overview
Structure and Expression
FGF-basic is a non-glycosylated, heparin-binding protein. The recombinant rat FGF-basic consists of 144 amino acids with a predicted molecular mass of approximately 16.2 kDa . It is expressed in almost all tissues, including the brain, pituitary, kidney, retina, bone, testis, adrenal gland, liver, monocytes, epithelial cells, and endothelial cells .
Biological Functions
FGF-basic plays a crucial role in various physiological and pathological processes:
- Cell Proliferation: It is mitogenic for many cell types, including epithelial and mesenchymal cells .
- Angiogenesis: FGF-basic shows potent angiogenic activity and has been implicated in tumor angiogenesis .
- Wound Healing: It significantly promotes the proliferation of adipose-derived mesenchymal cells and enhances chondrogenesis in three-dimensional micromass culture .
- Neurogenesis: In cultured astrocytes from rat brain, recombinant human basic fibroblast growth factor may induce nerve growth factor secretion .
Mechanism of Action
FGF-basic binds to a family of four distinct, high-affinity tyrosine kinase receptors, designated FGFR-1 to FGFR-4 . Additionally, it binds to the extracellular matrix (ECM) and heparan sulfate (HS), which are essential for FGF signaling . At the molecular level, two crystallographic models have been proposed to explain how heparan sulfate enables FGF and FGFR to assemble into a functional dimer on the cell surface .
Applications in Research
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