FGF 2 Human (147 a.a.)
Fibroblast Growth Factor Basic 147 a.a. Human Recombinant
Cat. No.
BT6532
Source
Escherichia Coli.
Synonyms
Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16.5kDa.
The FGF2 is purified by proprietary chromatographic techniques.
The FGF2 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Fibroblast growth factor-basic (FGF-basic), also known as FGF-2, is a member of the fibroblast growth factor (FGF) family. FGFs are involved in various biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. FGF-basic exhibits broad mitogenic and cell survival activities. It plays a role in modifying endothelial cell migration and proliferation and acts as an angiogenic factor. In vitro, it stimulates the mitosis of various mesoderm- and neuroectoderm-derived cells, suggesting its involvement in organogenesis. Three alternatively spliced variants of this protein exist, encoding different isoforms. Heparin-binding growth factors, including FGF-basic, are angiogenic agents in vivo and potent mitogens for diverse cell types in vitro. The tissue distribution and concentration of these growth factors can differ.
Description
Fibroblast Growth Factor-2 Human Recombinant (FGF-2) is produced in E. coli. It is a single, non-glycosylated polypeptide chain consisting of 147 amino acids, with a molecular weight of 16.5 kDa. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The bFGF was lyophilized from a sterile filtered solution containing 20mM Tris-HCl, pH 7.6, and 150mM NaCl.
Solubility
Reconstitute the lyophilized FGF-B in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized basic-FGF is stable at room temperature for 3 weeks. However, it is recommended to store the desiccated product below -18°C. Upon reconstitution, store FGFb at 4°C for 2-7 days. For long-term storage, aliquot and store below -18°C. It is advisable to add a carrier protein (0.1% HSA or BSA) for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Greater than 98.0% as determined by SDS-PAGE.
Biological Activity
The ED₅₀, as determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells, is less than 0.05 ng/ml. This corresponds to a specific activity of 2.0 x 10⁷ units/mg.
Synonyms
Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.
Source
Escherichia Coli.
Amino Acid Sequence
MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS.
Product Science Overview
Structure and Characteristics
-
Amino Acid Sequence: The 147 a.a. variant of FGF-2 has a molecular weight of approximately 16.5 kDa . The amino acid sequence is as follows:
MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS -
Isoforms: FGF-2 exists in multiple isoforms, with the 18 kDa isoform being predominantly cytosolic and acting through cell surface receptors. Other isoforms, such as the 22, 22.5, 24, and 34 kDa variants, are nuclear and may signal independently of transmembrane receptor pathways .
Biological Functions
FGF-2 is involved in a wide range of biological processes:
- Cell Proliferation: It stimulates the proliferation of various cell types, including mesenchymal, neuroectodermal, and endothelial cells .
- Angiogenesis: FGF-2 exerts potent angiogenic activity, promoting the formation of new blood vessels .
- Wound Healing: It plays a significant role in tissue repair and wound healing by promoting cell growth and tissue regeneration .
- Development: FGF-2 is crucial for limb and nervous system development .
Applications
Recombinant FGF-2 is widely used in research and therapeutic applications:
- Cell Culture: It is used to maintain and proliferate various cell lines in vitro .
- Tissue Engineering: FGF-2 is employed in tissue engineering and regenerative medicine to promote tissue repair and regeneration .
- Cancer Research: Due to its role in cell proliferation and angiogenesis, FGF-2 is studied in the context of tumor growth and cancer therapy .
Storage and Handling
- Reconstitution: The lyophilized protein should be reconstituted in sterile water at a concentration of 0.1 mg/mL. It is recommended to gently pipet the solution down the sides of the vial and avoid vortexing .
- Storage: Reconstituted FGF-2 should be stored at -80°C with the addition of 0.1% BSA for stability .
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
