For reconstitution of the lyophilized Fibroblast Growth Factor Basic, sterile 18MΩ-cm H2O at a concentration of 100µg/ml is recommended. This solution can be further diluted into other aqueous solutions as needed.
Fibroblast Growth Factor-Basic (FGF-b), also known as FGF-2, is a member of the fibroblast growth factor family, which plays a crucial role in various biological processes, including cell proliferation, differentiation, and tissue repair . The recombinant form of this protein, produced in plants, offers a promising alternative to traditional production methods due to its cost-effectiveness and scalability .
The production of human recombinant proteins in plants, also known as plant molecular farming, has gained significant attention due to its potential to produce high-value proteins efficiently . One of the key advantages of using plants as biofactories is the reduced risk of contamination by endotoxins or animal pathogens, which is a common concern with conventional expression platforms such as Escherichia coli or animal cell bioreactors .
In the case of FGF-b, researchers have successfully generated transplastomic tobacco lines that stably express the human FGF-b in their chloroplasts . These plants accumulate the recombinant protein in their leaves, which can then be purified and used for various applications .
The recombinant FGF-b produced in plants has been shown to retain its biological activity. For instance, it has demonstrated the ability to induce proliferation in HEK293T cell lines, which is a key indicator of its functionality . This makes plant-produced FGF-b a viable option for use in animal cell culture and regenerative medicine .
FGF-b has a wide range of applications in both research and clinical settings. It is used in cell culture to promote the growth and maintenance of various cell types, including mesenchymal, neuroectodermal, and endothelial cells . Additionally, FGF-b is known for its potent angiogenic activity, making it valuable in tissue engineering and regenerative medicine .