FCGR3A Human, Sf9
CD16a Human Recombinant, Sf9
Cat. No.
BT30990
Source
Sf9, Baculovirus cells.
Synonyms
Low affinity immunoglobulin gamma Fc region receptor III-A, CD16a antigen, Fc-gamma, RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2, CD16a, FCGR3A, FCG3, FCGR3, IGFR3, CD16, FCGRIII.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
FCGR3A Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 200 amino acids (18-208 a.a.) and having a molecular mass of 22.8kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
FCGR3A is fused with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
FCGR3A is fused with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Low affinity immunoglobulin gamma Fc region receptor III-A (FCGR3A), also known as CD16a, is a receptor that binds to the Fc portion of immunoglobulin G (IgG). It plays a crucial role in the immune system by participating in the removal of antigen-antibody complexes from circulation and other antibody-dependent immune responses. FCGR3A requires association with the gamma subunit of Fc epsilon for its function. Primarily expressed on natural killer (NK) cells, FCGR3A exists as an integral membrane glycoprotein anchored to the cell membrane via a transmembrane peptide. In contrast, FCGR3B is found on polymorphonuclear neutrophils (PMN) and is anchored through a phosphatidylinositol (PI) linkage. FCGR3A expression also occurs on macrophages, a subset of T cells, immature thymocytes, and placental trophoblasts. The receptor mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses such as phagocytosis. Genetic mutations in FCGR3A have been linked to increased susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.
Description
Recombinant FCGR3A protein, derived from humans and produced in Sf9 insect cells (Baculovirus expression system), is a single, glycosylated polypeptide chain. It comprises 200 amino acids (specifically, amino acids 18-208) and exhibits a molecular mass of 22.8 kDa. When analyzed using SDS-PAGE, the apparent molecular size falls within the range of 28-40 kDa due to glycosylation. The protein includes a 6 amino acid Histidine tag (His tag) fused at the C-terminus to facilitate purification, which is achieved through proprietary chromatographic methods.
Physical Appearance
A clear and colorless solution that has been sterilized by filtration.
Formulation
The FCGR3A protein solution is provided at a concentration of 0.25 mg/ml in a buffer consisting of phosphate-buffered saline (PBS) at pH 7.4 and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the unopened vial should be kept at 4°C. Long-term storage requires freezing at -20°C. The addition of a carrier protein (either HSA or BSA) at a concentration of 0.1% is recommended for extended storage. Repeated freezing and thawing of the protein solution should be avoided.
Purity
The purity of the FCGR3A protein is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
Low affinity immunoglobulin gamma Fc region receptor III-A, CD16a antigen, Fc-gamma, RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2, CD16a, FCGR3A, FCG3, FCGR3, IGFR3, CD16, FCGRIII.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADPMRTEDLP KAVVFLEPQW YRVLEKDSVT LKCQGAYSPE DNSTQWFHNE SLISSQASSY FIDAATVDDS GEYRCQTNLS TLSDPVQLEV HIGWLLLQAP RWVFKEEDPI HLRCHSWKNT ALHKVTYLQN GKGRKYFHHN SDFYIPKATL KDSGSYFCRG LFGSKNVSSE TVNITITQGL AVSTISSFFP PGYQHHHHHH.
Product Science Overview
Structure and Expression
CD16a is a glycoprotein that is primarily expressed on the surface of natural killer (NK) cells, macrophages, and a subset of T cells . It is a low/intermediate affinity receptor for polyvalent immune-complexed IgG . The human recombinant version of CD16a is produced in Sf9 Baculovirus cells, which allows for the expression of a single, glycosylated polypeptide chain containing 200 amino acids (18-208 a.a.) with a molecular mass of approximately 22.8 kDa .
Function
CD16a is involved in several key immune functions:
- Antibody-Dependent Cellular Cytotoxicity (ADCC): CD16a binds to the Fc region of IgG antibodies that are attached to target cells, leading to the activation of NK cells and the subsequent destruction of the target cells .
- Phagocytosis: Macrophages expressing CD16a can engulf and digest antibody-coated pathogens .
- Secretion of Enzymes and Inflammatory Mediators: Upon activation, cells expressing CD16a can release various enzymes and cytokines that contribute to the immune response .
Applications in Research and Medicine
Recombinant CD16a is widely used in research to study immune responses and to develop therapeutic antibodies. The humanized CD16a model has been employed to evaluate the efficacy of antibody therapeutics in triggering ADCC, which is a critical mechanism for the effectiveness of many cancer immunotherapies .
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