FCGR3A Human, HEK
HEK293 Cells
Description
FCGR3A Human Recombinant produced HEK293 Cells is a single, polypeptide chain containing 197 amino acids (18-208 a.a) and having a molecular mass of 22.6 kDa. FCGR3A is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
HEK293 Cells
MRTEDLPKAV VFLEPQWYRV LEKDSVTLKC QGAYSPEDNS TQWFHNESLI SSQASSYFID AATVDDSGEY RCQTNLSTLS DPVQLEVHIG WLLLQAPRWV FKEEDPIHLR CHSWKNTALH KVTYLQNGKG RKYFHHNSDF YIPKATLKDS GSYFCRGLFG SKNVSSETVN ITITQGLAVS TISSFFPPGY Q HHHHHH.
Product Science Overview
CD16a is encoded by the FCGR3A gene and is expressed on the surface of several immune cells, including natural killer (NK) cells, macrophages, a subset of T-cells, immature thymocytes, and placental trophoblasts . It is a transmembrane glycoprotein that is anchored to the cell membrane via a polypeptide chain. The receptor has an intermediate affinity for IgG antibodies, which allows it to bind to the Fc region of these antibodies and mediate immune responses .
The recombinant form of CD16a is often expressed in HEK 293 cells (Human Embryonic Kidney 293 cells), which are widely used in biological research due to their high transfection efficiency and reliable protein expression . The recombinant protein is typically tagged with a 6-His tag at the C-terminus to facilitate purification and detection .
CD16a is involved in several critical immune functions:
- Phagocytosis: CD16a mediates the engulfment and digestion of pathogens and cellular debris by immune cells.
- Secretion of Enzymes and Inflammatory Mediators: Upon activation, CD16a triggers the release of enzymes and inflammatory molecules that help in the immune response.
- Antibody-Dependent Cellular Cytotoxicity (ADCC): CD16a plays a key role in ADCC, where NK cells recognize and kill antibody-coated target cells.
- Clearance of Immune Complexes: CD16a helps in the removal of immune complexes from the circulation, preventing their deposition in tissues and subsequent inflammation .
Aberrant expression or mutations in CD16a have been implicated in various diseases and conditions, including:
- Recurrent Viral Infections: Defective CD16a function can lead to increased susceptibility to viral infections.
- Systemic Lupus Erythematosus (SLE): Altered CD16a expression is associated with the autoimmune disease SLE.
- Alloimmune Neonatal Neutropenia: Mutations in CD16a can cause this condition, characterized by low levels of neutrophils in newborns .
Recombinant CD16a expressed in HEK 293 cells is widely used in research to study its structure, function, and role in immune responses. The recombinant protein is typically lyophilized from a filtered solution in phosphate-buffered saline (PBS) with added protectants like mannitol or trehalose . It can be reconstituted in sterile PBS for experimental use and stored at -20°C for extended periods .
