FCGR3A Human

CD16a Human Recombinant
Cat. No.
BT30980
Source
E.coli.
Synonyms
Low affinity immunoglobulin gamma Fc region receptor III-A, CD16a antigen, Fc-gamma, RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2, CD16a, FCGR3A, FCG3, FCGR3, IGFR3, CD16, FCGRIII.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

FCGR3A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 228 amino acids (18-208 a.a) and having a molecular mass of 26kDa.
FCGR3A is fused to a 37 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Low affinity immunoglobulin gamma Fc region receptor III-A (FCGR3A), also known as CD16a, is a receptor for the Fc portion of immunoglobulin G (IgG). It plays a crucial role in the immune system by mediating antibody-dependent responses, such as antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis. FCGR3A is expressed on various immune cells, including natural killer (NK) cells, macrophages, and a subset of T cells. It requires association with the gamma subunit of Fc epsilon for its function. Genetic variations in FCGR3A have been linked to susceptibility to autoimmune diseases and viral infections.
Description
Recombinant human FCGR3A protein was produced in E. coli and purified to a high degree. It is a single, non-glycosylated polypeptide chain that contains 228 amino acids (including a 37 amino acid His-tag at the N-terminus) and has a molecular mass of 26 kDa. The protein sequence spans amino acids 18-208 of the mature FCGR3A protein.
Physical Appearance
Clear and colorless solution after sterilization by filtration.
Formulation
The FCGR3A protein is provided as a 1 mg/ml solution in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 1 M urea, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), store the protein solution at 4°C. For long-term storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein such as HSA or BSA (0.1%) can help to preserve protein stability during long-term storage. Avoid repeated freezing and thawing of the protein solution.
Purity
The purity of the FCGR3A protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Low affinity immunoglobulin gamma Fc region receptor III-A, CD16a antigen, Fc-gamma, RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2, CD16a, FCGR3A, FCG3, FCGR3, IGFR3, CD16, FCGRIII.
Source
E.coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRT EDLPKAVVFL EPQWYRVLEK DSVTLKCQGA YSPEDNSTQW FHNESLISSQ ASSYFIDAAT VDDSGEYRCQ TNLSTLSDPV QLEVHIGWLL LQAPRWVFKE EDPIHLRCHS WKNTALHKVT YLQNGKGRKY FHHNSDFYIP KATLKDSGSY FCRGLFGSKN VSSETVNITI TQGLAVSTIS SFFPPGYQ.

Product Science Overview

Structure and Isoforms

CD16a is encoded by the FCGR3A gene and is expressed on the surface of natural killer (NK) cells, macrophages, and a subset of T cells. It is a transmembrane protein that consists of an extracellular domain, a transmembrane domain, and a cytoplasmic tail. The extracellular domain is responsible for binding to the Fc region of IgG, while the cytoplasmic tail is involved in signal transduction .

There are two isoforms of CD16: CD16a and CD16b. CD16a is expressed on NK cells and macrophages, while CD16b is expressed on neutrophils. The two isoforms differ in their membrane anchoring; CD16a is anchored via a transmembrane domain, whereas CD16b is anchored via a glycosylphosphatidylinositol (GPI) linkage .

Function

CD16a plays a pivotal role in the immune response by mediating ADCC, a mechanism through which NK cells lyse target cells that are coated with antibodies. When CD16a binds to the Fc region of IgG on the surface of target cells, it triggers the release of cytotoxic granules from NK cells, leading to the destruction of the target cells .

Additionally, CD16a is involved in the phagocytosis of antibody-coated pathogens by macrophages. Upon binding to immune complexes, CD16a activates signaling pathways that result in the engulfment and degradation of the pathogens .

Recombinant CD16a

Recombinant CD16a is a laboratory-produced version of the natural receptor, created using recombinant DNA technology. It is typically produced in mammalian cell lines, such as Chinese hamster ovary (CHO) cells, to ensure proper folding and post-translational modifications. Recombinant CD16a is used in various research applications, including studies on ADCC, immune complex clearance, and receptor-ligand interactions .

Applications

Recombinant CD16a is widely used in immunological research to study the mechanisms of ADCC and other Fc receptor-mediated processes. It is also employed in the development of therapeutic antibodies, as understanding the interaction between CD16a and IgG can help optimize antibody design for enhanced efficacy .

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