Eotaxin Rhesus Macaque

The CCL11 gene is encoded on three exons and is located on chromosome 17 in humans . The recombinant form of CCL11 from Rhesus Macaque is a single non-glycosylated polypeptide chain containing 74 amino acids . This recombinant protein is expressed in E. coli and purified using high-performance liquid chromatography (HPLC) and validated for bioactivity .

CCL11 selectively recruits eosinophils by inducing their chemotaxis, which is the movement of cells in response to chemical stimuli . This chemotactic activity is mediated through its binding to G-protein-linked receptors, specifically chemokine receptors such as CCR2, CCR3, and CCR5 . The biological activity of recombinant CCL11 is determined by a chemotaxis bioassay using human peripheral blood eosinophils, with effective concentrations ranging from 0.1 to 10.0 ng/mL .

Recombinant Rhesus Macaque CCL11 is used in various research applications, including studies on allergic responses and eosinophil-related diseases . The protein is highly pure, with a purity greater than 98% as determined by SDS-PAGE and HPLC . It is typically lyophilized and should be stored at -20 to -70°C for long-term storage .

The role of CCL11 in allergic responses makes it a significant target for research in conditions such as asthma, allergic rhinitis, and other eosinophil-associated diseases . By understanding the mechanisms through which CCL11 operates, researchers can develop better therapeutic strategies to manage and treat these conditions.