Eotaxin Human
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
The CCL11 is purified by proprietary chromatographic techniques.
Product Specs
GPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDICADPKKKWVQD
SMKYLDQKSP TPKP.
Product Science Overview
Eotaxin was first identified in guinea pigs as a protein responsible for attracting eosinophils to sites of inflammation. It is produced by various cell types, including endothelial cells, epithelial cells, and fibroblasts, in response to inflammatory stimuli such as interleukin-4 (IL-4) and tumor necrosis factor-alpha (TNF-α) .
The human recombinant form of Eotaxin (CCL11) is a small protein with a molecular weight of approximately 8.3 kDa. It consists of 74 amino acid residues, including four highly conserved cysteine residues that are characteristic of CC chemokines .
Eotaxin exerts its effects by binding to the CCR3 receptor on the surface of eosinophils. This interaction triggers a signaling cascade that leads to the migration of eosinophils to the site of inflammation. Eotaxin, along with its related chemokines Eotaxin-2 (CCL24) and Eotaxin-3 (CCL26), plays a key role in the regulation of eosinophil recruitment in the asthmatic lung and during allergic reactions .
Eotaxin is primarily associated with conditions involving eosinophilic inflammation, such as asthma, allergic rhinitis, and eosinophilic esophagitis. Elevated levels of Eotaxin have been detected in the bronchoalveolar lavage fluid and serum of patients with asthma, indicating its role in the pathogenesis of this disease .
In addition to its role in allergic diseases, recent studies have suggested that Eotaxin may also be involved in other conditions, such as psychiatric disorders. For example, elevated levels of Eotaxin have been observed in patients with depression and schizophrenia, although the exact mechanisms underlying these associations are still under investigation .
Recombinant human Eotaxin (CCL11) is produced using Escherichia coli (E. coli) expression systems. The recombinant protein is purified to high levels of purity, typically greater than 97%, and is used in various research applications, including studies on eosinophil biology, chemotaxis, and inflammation .
The recombinant form of Eotaxin is available in both carrier-free and carrier-containing formulations. The carrier-free version is recommended for applications where the presence of carrier proteins, such as bovine serum albumin (BSA), could interfere with experimental results .
