Eotaxin Mouse

Eotaxin is a protein with a molecular mass of approximately 8.4 kDa . It is constitutively expressed in the thymus and can be inducibly expressed in various tissues, including the lung, intestine, heart, spleen, and kidney . The recombinant form of mouse CCL11/Eotaxin is typically produced in Escherichia coli (E. coli) and is purified to a high degree of purity, often greater than 97% as determined by SDS-PAGE under reducing conditions .

The primary function of Eotaxin is to act as a potent chemoattractant for eosinophils . It binds to the CCR3 receptor on the surface of eosinophils, inducing their migration to sites of inflammation or allergic reactions . This process is essential for the body’s defense mechanisms against parasitic infections and in the pathophysiology of allergic diseases such as asthma and allergic rhinitis.

Recombinant mouse CCL11/Eotaxin is widely used in research to study its role in immune responses and its potential therapeutic applications. It is utilized in various assays to measure its ability to chemoattract cells, particularly in studies involving eosinophil-related diseases . The recombinant protein is available in different formulations, with or without carrier proteins like Bovine Serum Albumin (BSA), depending on the specific requirements of the experimental setup .

The recombinant mouse CCL11/Eotaxin protein is typically lyophilized and can be reconstituted in sterile PBS (Phosphate-Buffered Saline) for use in experiments . It is recommended to store the protein at -20 to -70°C to maintain its stability and avoid repeated freeze-thaw cycles . Under sterile conditions, the reconstituted protein can be stored for up to one month at 2 to 8°C or for up to three months at -20 to -70°C .