Eotaxin Rat

Eotaxin Rat Recombinant (CCL11)
Cat. No.
BT11908
Source
Escherichia Coli.
Synonyms
Small inducible cytokine A11, CCL11, Eosinophil chemotactic protein, chemokine (C-C motif) ligand 11, SCYA11.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USEONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Eotaxin Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 74 amino acids and having a molecular mass of 8.4kDa. The CCL11 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Chemokine (C-C motif) ligand 11 (CCL11), also known as eotaxin, is a small cytokine of the CC chemokine family. It plays a crucial role in allergic responses by selectively attracting eosinophils through chemotaxis. CCL11 exerts its effects by binding to a G-protein-coupled receptor called a chemokine receptor. CCL11 can bind to several chemokine receptors, including CCR2, CCR3, and CCR5. In humans, the gene responsible for encoding CCL11 (scya11) consists of three exons and is situated on chromosome 17.
Description
Recombinant Rat Eotaxin, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 74 amino acids. It has a molecular weight of 8.4 kDa. The purification process for CCL11 involves the use of proprietary chromatographic methods.
Physical Appearance
White, sterile-filtered lyophilized (freeze-dried) powder.
Formulation
The product is filtered through a 0.2µm filter and lyophilized from a concentrated solution (1mg/ml) in 1xPBS with a pH of 7.4.
Solubility
To reconstitute the lyophilized Eotaxin, it is recommended to dissolve it in sterile 18M-cm H₂O at a concentration of at least 100µg/ml. This reconstituted solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Eotaxin, while stable at room temperature for up to 3 weeks, should ideally be stored in a dry environment below -18°C. After reconstitution, CCL11 should be kept at 4°C for a period of 2-7 days. For long-term storage, freezing below -18°C is recommended. To enhance stability during long-term storage, consider adding a carrier protein like HSA or BSA (0.1%). It is important to avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 97.0% through the following methods: (a) Analysis using RP-HPLC. (b) Analysis using SDS-PAGE.
Biological Activity
The biological activity is assessed by evaluating the ability of the product to induce chemotaxis in purified blood eosinophils. This is determined using a concentration range of 0.1-1.0 µg/ml. This corresponds to a specific activity ranging from 10-100IU/mg.
Synonyms
Small inducible cytokine A11, CCL11, Eosinophil chemotactic protein, chemokine (C-C motif) ligand 11, SCYA11.
Source
Escherichia Coli.
Amino Acid Sequence
HPGSIPTSCC FTMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTKLGKEICADPKKKWVQD ATKHLDQKLQ TPKP.

Product Science Overview

Discovery and Structure

Eotaxin was first identified in guinea pigs, where it was purified from bronchoalveolar lavage fluid following an allergic challenge with ovalbumin . The protein is composed of approximately 74 amino acids and has a molecular weight of around 8.4 kDa . The recombinant form of rat CCL11 is produced using E. coli expression systems, ensuring high purity and activity .

Function and Mechanism

Eotaxin is a potent chemoattractant for eosinophils, a type of white blood cell involved in allergic reactions and asthma . It binds to the CCR3 receptor on the surface of eosinophils, triggering their migration to the site of inflammation . Unlike other chemokines, eotaxin is highly specific for eosinophils and does not attract monocytes or neutrophils .

Role in Diseases

Eotaxin is implicated in various allergic conditions, including asthma, allergic rhinitis, and atopic dermatitis . Elevated levels of eotaxin are often found in the tissues and blood of individuals with these conditions. Additionally, recent research has highlighted its role in neuroinflammatory and neurodegenerative disorders. Increased levels of eotaxin have been associated with cognitive impairments and accelerated brain aging .

Applications in Research

Recombinant eotaxin is widely used in research to study its role in immune responses and disease mechanisms. It is also utilized in assays to evaluate the efficacy of anti-inflammatory drugs and to understand the pathways involved in eosinophil recruitment .

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