ENO1 Mouse
Escherichia Coli.
0610008I15, AL022784, Eno-1, MBP-1, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 1, Non-neural enolase, NNE, Eno1.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
ENO1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 457 amino acids (1-434 a.a) and having a molecular mass of 24kDa.
ENO1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
0610008I15, AL022784, Eno-1, MBP-1, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 1, Non-neural enolase, NNE, Eno1.
Escherichia Coli.
MGSSHHHHHH SSGLVPRGSH MGSMSILRIH AREIFDSRGN PTVEVDLYTA KGLFRAAVPS GASTGIYEAL ELRDNDKTRF MGKGVSQAVE HINKTIAPAL VSKKVNVVEQ EKIDKLMIEM DGTENKSKFG ANAILGVSLA VCKAGAVEKG VPLYRHIADL AGNPEVILPV PAFNVINGGS
HAGNKLAMQE FMILPVGASS FREAMRIGAE VYHNLKNVIK EKYGKDATNV GDEGGFAPNI LENKEALELL KTAIAKAGYT DQVVIGMDVA ASEFYRSGKY DLDFKSPDDP SRYITPDQLA DLYKSFVQNY PVVSIEDPFD QDDWGAWQKF TASAGIQVVG DDLTVTNPKR IAKAASEKSC
NCLLLKVNQI GSVTESLQAC KLAQSNGWGV MVSHRSGETE DTFIADLVVG LCTGQIKTGA PCRSERLAKY NQILRIEEEL GSKAKFAGRS FRNPLAK
Product Science Overview
Enolase-1 is one of three enolase isoenzymes found in mammals, the others being enolase-2 (neuron-specific enolase) and enolase-3 (muscle-specific enolase) . Enolase-1 is a homodimeric soluble enzyme, meaning it forms a dimer consisting of two identical subunits. Additionally, it encodes a shorter monomeric structural lens protein known as tau-crystallin . The full-length protein is found in the cytoplasm, while the shorter protein is produced from an alternative translation start and is localized to the nucleus .
Recombinant Enolase-1 (Mouse) is typically produced using Escherichia coli (E. coli) expression systems . The recombinant protein often includes an N-terminal His-tag to facilitate purification. The amino acid sequence of the recombinant mouse Enolase-1 corresponds to the amino acids 1-434 of the native protein . The recombinant protein is bioactive and retains its enzymatic activity, which is crucial for various biochemical and research applications .
Enolase-1 is involved in several biological processes beyond glycolysis. It acts as a plasminogen receptor on the cell surface, particularly in tumors, contributing to cancer cell proliferation, migration, invasion, and metastasis . This “moonlighting” function of Enolase-1 highlights its role in various cellular processes, including immune responses and tissue remodeling .
Due to its involvement in glycolysis and its role as a plasminogen receptor, Enolase-1 is considered a potential biomarker for various diseases, including cancer . Its expression levels can be indicative of tumor progression and metastasis, making it a target for diagnostic and therapeutic interventions .
Recombinant Enolase-1 (Mouse) is widely used in research to study its enzymatic activity, structure-function relationships, and its role in various biological processes. It is also used in the development of diagnostic assays and therapeutic strategies targeting Enolase-1 in diseases such as cancer .
