ENO1 Human

Enolase-1 Human Recombinant
Cat. No.
BT16508
Source
Escherichia Coli.
Synonyms
NNE, PPH, MPB1, MBP-1, ENO1L1, ENO1, Alpha-Enolase, Enolase-Alpha, 2-phospho-D-glycerate hydro-lyase, Non-neural enolase, Enolase 1, MPB-1, Phosphopyruvate hydratase, C-myc promoter-binding protein, Plasminogen-binding protein, MBPB1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

The ENO1 Human Recombinant protein is a single, non-glycosilated polypeptide chain produced in E. coli, having a molecular weight of 47.1kDa and containing 434 amino acids (1-434 a.a.).

Product Specs

Introduction
ENO1, also known as Enolase-1, is a crucial glycolytic enzyme found in nearly all tissues. This homodimeric protein exists in two forms: a cytoplasmic full-length form and a shorter nuclear form. The full-length ENO1 participates in glycolysis, particularly under hypoxic conditions, and acts as a plasminogen receptor on cell surfaces during tissue invasion. The shorter form is involved in the regulation of the c-myc promoter. Deregulated ENO1 expression is implicated in the progression of several cancers, including breast and lung cancer. Furthermore, ENO1 serves as an autoantigen in autoimmune diseases such as Hashimoto's encephalopathy and severe asthma. In Behcet's disease, ENO1 is targeted by serum anti-endothelial antibodies.
Description
This product consists of the recombinant human ENO1 protein, produced in E. coli. This non-glycosylated protein is a single polypeptide chain with a molecular weight of 47.1kDa, encompassing amino acids 1 to 434.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The ENO1 protein is provided at a concentration of 1mg/ml in a solution containing 20mM Tris-HCl (pH 7.5), 1mM MgSO4, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), keep the vial refrigerated at 4°C. For extended storage, freeze the protein at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Biological Activity
The specific activity of this protein is measured as greater than 20,000 pmol/min/ug. This value is determined by monitoring the decrease in NAD absorbance at 340nm, which corresponds to the formation of NADH at pH 6.5 and 37°C.
Purity
The purity of this protein is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
NNE, PPH, MPB1, MBP-1, ENO1L1, ENO1, Alpha-Enolase, Enolase-Alpha, 2-phospho-D-glycerate hydro-lyase, Non-neural enolase, Enolase 1, MPB-1, Phosphopyruvate hydratase, C-myc promoter-binding protein, Plasminogen-binding protein, MBPB1.
Source
Escherichia Coli.
Amino Acid Sequence
MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK.

Product Science Overview

Structure and Function

Enolase-1 is a multifunctional enzyme with a molecular mass of approximately 49.3 kDa . It catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, a critical step in glycolysis. Beyond its glycolytic function, Enolase-1 also acts as a plasminogen receptor on the cell surface, particularly in tumors, where it contributes to cancer cell proliferation, migration, invasion, and metastasis .

Biological Significance

Enolase-1 is involved in various physiological and pathological processes. It is predominantly expressed during the early stages of embryonic development . In cancer biology, Enolase-1 overexpression and post-translational modifications are of diagnostic and prognostic value in many cancer types . The enzyme’s role in anaerobic metabolism under hypoxic conditions makes it a critical player in tumor progression and survival .

Recombinant Enolase-1

Recombinant human Enolase-1 is produced using DNA sequences encoding the human ENO1 gene, typically expressed in Escherichia coli . The recombinant protein is often tagged with polyhistidine for purification purposes and is verified for purity using techniques like SDS-PAGE and HPLC . It is commonly used in research to study its biochemical properties, interactions, and potential as a therapeutic target.

Applications in Research

Recombinant Enolase-1 is valuable in various research applications, including:

  • Cancer Research: Studying its role in tumor biology and as a potential biomarker for cancer diagnosis and prognosis .
  • Immunology: Investigating its interaction with immune cells, such as CD4+ T cells, and its impact on immune responses .
  • Metabolic Studies: Exploring its function in glycolysis and its regulation under different physiological conditions .
Storage and Stability

Recombinant Enolase-1 is typically lyophilized and stored at -20°C to -80°C to maintain its stability and activity. It is recommended to avoid repeated freeze-thaw cycles to preserve its integrity .

Enolase-1’s multifunctional nature and involvement in critical biological processes make it a significant focus of research, particularly in understanding cancer biology and developing potential therapeutic interventions.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

ENO1 Mouse
Enolase-1 Mouse Recombinant
Cat. No.
BT16585
Source
<p>Escherichia Coli.</p>
ENO1 Antibody
Enolase-1, Mouse Anti Human
Cat. No.
BT16699
Source
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.