ENO1 Antibody
Description
Product Specs
Product Science Overview
Enolase-1, also known as alpha-enolase or ENO1, is a glycolytic enzyme that plays a crucial role in the glycolysis pathway by catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate. This enzyme is encoded by the ENO1 gene and is ubiquitously expressed in various tissues . Enolase-1 is one of three enolase isoforms, with the other two being gamma-enolase (ENO2) and beta-enolase (ENO3), which are expressed in neuronal cells and muscle tissues, respectively .
Enolase-1 has gained significant attention in cancer research due to its overexpression in a wide range of human cancers. This overexpression is often associated with poor prognosis and increased tumor aggressiveness . Enolase-1 functions as a plasminogen receptor on the cell surface, particularly in tumors, facilitating cancer cell proliferation, migration, invasion, and metastasis . Additionally, Enolase-1 promotes oncogenic events such as protein-protein interactions that regulate glycolysis, activation of signaling pathways, and resistance to chemotherapy .
The mouse anti-human Enolase-1 antibody is a monoclonal antibody specifically designed to target and bind to the Enolase-1 protein in human cells. This antibody is widely used in research and diagnostic applications to study the expression and function of Enolase-1 in various biological processes and diseases, particularly cancer .
The mouse anti-human Enolase-1 antibody has been instrumental in cancer research for several reasons:
- Detection and Quantification: It is used in techniques such as Western blotting, immunohistochemistry, and ELISA to detect and quantify Enolase-1 expression in cancer tissues and cell lines .
- Therapeutic Targeting: Researchers have explored the potential of targeting Enolase-1 with specific antibodies to inhibit tumor growth and metastasis. For example, studies have shown that blocking surface Enolase-1 with monoclonal antibodies can reduce tumor growth, angiogenesis, and inflammation in prostate cancer models .
- Biomarker Development: Enolase-1 is considered a promising cancer biomarker due to its overexpression in tumors and the presence of anti-Enolase-1 autoantibodies in some cancer patients . This makes it a valuable target for developing diagnostic and prognostic tools.
