E Selectin Human
E-selectin Human Recombinant
Cat. No.
BT21132
Source
Escherichia Coli.
Synonyms
E-selectin, Endothelial leukocyte adhesion molecule 1, ELAM-1, Leukocyte-endothelial cell adhesion molecule 2, LECAM2, CD62E antigen, SELE, ELAM1, ELAM, ESEL, CD62E.
Appearance
Sterile Filtered liquid formulation.
Purity
Greater than 95% as determined by SDS-PAGE.
Single band on Western Blot.
Single band on Western Blot.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
E-Selectin Human Recombinant is expressed in E. coli containing amino acids 179-557 fused to an amino terminal hexahistidine tag, having a total molecular weight of 45.22 kDa.
Product Specs
Introduction
E-selectin, also known as Endothelial leukocyte adhesion molecule 1 (ELAM1 or ELAM), is a member of the selectin family of adhesion molecules. These molecules are glycoproteins that bind to carbohydrates in a calcium-dependent manner. E-selectin is found on the surface of endothelial cells, which line the inside of blood vessels. It plays a crucial role in inflammation by facilitating the adhesion of leukocytes (white blood cells) and platelets to endothelial cells. This interaction is a critical early step in the inflammatory response, allowing leukocytes to migrate from the bloodstream into surrounding tissues. The gene encoding E-selectin is present in a single copy in the human genome and consists of 14 exons distributed over approximately 13 kilobases of DNA.
Description
Recombinant Human E-Selectin is produced in E. coli. This protein encompasses amino acid residues 179 to 557 and includes an amino-terminal hexahistidine tag, resulting in a total molecular weight of 45.22 kDa.
Physical Appearance
The product is a sterile-filtered liquid.
Formulation
E-Selectin is supplied in a solution containing 1x PBS (phosphate-buffered saline) and 50% glycerol.
Stability
For short-term storage (1-2 weeks), keep the vial refrigerated at 4°C. For long-term storage, freeze the product at -20°C. To maintain product integrity, minimize repeated freeze-thaw cycles.
Purity
The purity of E-Selectin is greater than 95%, as determined by SDS-PAGE analysis. The protein appears as a single band on Western Blot.
Synonyms
E-selectin, Endothelial leukocyte adhesion molecule 1, ELAM-1, Leukocyte-endothelial cell adhesion molecule 2, LECAM2, CD62E antigen, SELE, ELAM1, ELAM, ESEL, CD62E.
Source
Escherichia Coli.
Product Science Overview
Structure and Function
E-selectin is a transmembrane protein composed of several distinct domains:
- N-terminal C-type lectin domain: This domain is responsible for binding to specific carbohydrate structures on the surface of leukocytes.
- Epidermal Growth Factor (EGF)-like domain: This domain is involved in protein-protein interactions.
- Sushi domains (SCR repeats): These domains contribute to the structural stability of the protein.
- Transmembrane domain: This domain anchors the protein to the cell membrane.
- Intracellular cytoplasmic tail: This domain is involved in intracellular signaling.
E-selectin is transiently expressed on the surface of endothelial cells in response to pro-inflammatory cytokines such as interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α). Its expression peaks around 4 hours after stimulation and declines within 24 hours .
Role in Inflammation
E-selectin plays a pivotal role in the recruitment of leukocytes to sites of inflammation. The process involves several steps:
- Rolling: Leukocytes in the bloodstream initially make loose, transient contacts with the endothelium through interactions between E-selectin and its carbohydrate ligands on leukocytes.
- Activation: Chemokines and other signaling molecules activate leukocytes, leading to conformational changes in integrins on their surface.
- Firm Adhesion: Activated integrins on leukocytes bind firmly to their ligands on the endothelium, allowing leukocytes to adhere strongly and eventually transmigrate through the endothelial layer to reach the site of inflammation.
Recombinant Human E-selectin
Recombinant human E-selectin is produced using genetic engineering techniques. The gene encoding E-selectin is inserted into a suitable expression system, such as Chinese Hamster Ovary (CHO) cells, which then produce the protein. The recombinant protein is purified to high levels of purity and can be used for various research and therapeutic applications .
Applications
Recombinant human E-selectin is used in several research areas, including:
- Inflammation studies: Understanding the mechanisms of leukocyte recruitment and adhesion.
- Drug development: Screening for potential anti-inflammatory drugs that target E-selectin-mediated pathways.
- Cell adhesion assays: Investigating the interactions between leukocytes and endothelial cells.
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