L Selectin Human
L-selectin Human Recombinant
Cat. No.
BT21189
Source
Escherichia Coli.
Synonyms
L-selectin, Lymph node homing receptor, Leukocyte adhesion molecule 1, LAM-1, Leukocyte surface antigen Leu-8, TQ1, gp90-MEL, Leukocyte-endothelial cell adhesion molecule 1, LECAM1, CD62 antigen-like family member L, CD62L antigen, LAM1, LNHR, LSEL, CD62L, LYAM1, Leu-8, PLNHR, hLHRc, Lyam-1, L-Sel.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Single band on Western Blot.
Single band on Western Blot.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
L-Selectin Human Recombinant is expressed in E. coli containing 294 amino acids 39-332 fused to an amino terminal hexahistidine tag, having a total molecular weight of 37.55kDa.
Product Specs
Introduction
L-Selectin, a divalent cation-dependent carbohydrate-binding glycoprotein, plays a crucial role in cell adhesion. Its structure comprises domains homologous to lectins, epidermal growth factor, and C3/C4 binding proteins. Expressed on lymphocytes, monocytes, and granulocytes, L-Selectin binds to carbohydrates on activated endothelial cells. Shedding via proteolytic cleavage allows circulating L-Selectin levels to serve as indicators of pathological conditions. Notably, ADAM17 is responsible for L-Selectin cleavage. Functioning as a "homing receptor," L-Selectin guides leukocytes into secondary lymphoid tissues through high endothelial venules. This interaction facilitates leukocyte localization at specific sites. Additionally, naive T cells, prior to antigen encounter, display L-Selectin on their surfaces, which disappears upon activation.
Description
Recombinant Human L-Selectin, expressed in E. coli, consists of 294 amino acids (residues 39-332) and a hexahistidine tag at the N-terminus, resulting in a 37.55 kDa protein.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The L-Selectin protein is supplied in a buffer containing 1x PBS and 50% Glycerol.
Stability
For short-term storage (1-2 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Repeated freezing and thawing should be avoided.
Purity
The purity of L-Selectin is greater than 95% as determined by SDS-PAGE analysis, showing a single band on Western Blot.
Applications
This L-Selectin protein is suitable as a positive control in various immunological techniques, including Western blotting, ELISA, and immunoprecipitation. However, its biological activity has not been verified yet.
Synonyms
L-selectin, Lymph node homing receptor, Leukocyte adhesion molecule 1, LAM-1, Leukocyte surface antigen Leu-8, TQ1, gp90-MEL, Leukocyte-endothelial cell adhesion molecule 1, LECAM1, CD62 antigen-like family member L, CD62L antigen, LAM1, LNHR, LSEL, CD62L, LYAM1, Leu-8, PLNHR, hLHRc, Lyam-1, L-Sel.
Source
Escherichia Coli.
Product Science Overview
Structure and Function
L-Selectin is a calcium-dependent lectin that plays a crucial role in the initial adhesive interactions between leukocytes and endothelial cells during inflammation and immune surveillance . It mediates the tethering and rolling of leukocytes on the vascular endothelium, which is a prerequisite for their subsequent firm adhesion and transmigration into tissues .
Molecular Characteristics
The recombinant human L-Selectin protein is typically produced in Chinese Hamster Ovary (CHO) cell lines . The protein has a predicted molecular mass of approximately 33 kDa, but it appears larger (50-70 kDa) on SDS-PAGE due to glycosylation . The protein is often tagged with a His-tag or Fc Chimera to facilitate purification and detection .
Applications
Recombinant L-Selectin is used in various research applications, including:
- Cell adhesion studies: Understanding the mechanisms of leukocyte-endothelial interactions.
- Inflammation research: Investigating the role of L-Selectin in inflammatory diseases.
- Drug development: Screening for potential inhibitors of L-Selectin-mediated adhesion.
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