L-Selectin Human, Sf9
Sf9, Baculovirus cells.
Selectin L, Leukocyte-Endothelial Cell Adhesion Molecule 1, CD62 Antigen-Like Family Member L, Leukocyte Surface Antigen Leu-8, Lymphocyte Adhesion Molecule 1, Lymph Node Homing Receptor, Gp90-MEL, LECAM1, LYAM1, LNHR, TQ1, Leukocyte Adhesion Molecule, Pln Homing Receptor, CD62L Antigen, L-Selectin, CD62L, PLNHR, LAM-1, LAM1, LEU8, LSEL, L-selectin.
Greater than 90% as determined by SDS-PAGE.
Description
L-Selectin produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 303 amino acids (52-345a.a.) and having a molecular mass of 34.1kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
L-Selectin is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
L-Selectin, a member of the selectin family of adhesion molecules, plays a crucial role in leukocyte trafficking and immune responses. This glycoprotein facilitates the initial interaction of leukocytes with endothelial cells, guiding their migration to sites of inflammation or lymphoid tissues. L-Selectin's structure comprises distinct domains, including a lectin domain for carbohydrate binding, an epidermal growth factor-like domain, and complement control protein-like repeats. Expressed on various immune cells like lymphocytes, monocytes, and granulocytes, L-Selectin binds to specific carbohydrate ligands on activated endothelial cells, mediating leukocyte rolling and adhesion. Its expression levels are dynamically regulated, with shedding occurring upon cellular activation, making it a potential marker for immune activation and disease states. Notably, L-Selectin is cleaved by the enzyme ADAM17, highlighting its tightly regulated function in immune homeostasis.
Recombinant L-Selectin, expressed in Sf9 insect cells using a baculovirus system, is a single, glycosylated polypeptide chain. This protein encompasses amino acids 52-345, corresponding to the extracellular domain, with a molecular weight of approximately 34.1 kDa. Due to glycosylation, it appears as a band around 40-57 kDa on SDS-PAGE. The recombinant protein includes a 6-amino acid Histidine tag at the C-terminus to facilitate purification by affinity chromatography.
The L-Selectin protein is supplied as a solution at a concentration of 0.25 mg/ml in a buffer consisting of Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
The purity of the L-Selectin protein is determined to be greater than 90% using SDS-PAGE analysis.
Selectin L, Leukocyte-Endothelial Cell Adhesion Molecule 1, CD62 Antigen-Like Family Member L, Leukocyte Surface Antigen Leu-8, Lymphocyte Adhesion Molecule 1, Lymph Node Homing Receptor, Gp90-MEL, LECAM1, LYAM1, LNHR, TQ1, Leukocyte Adhesion Molecule, Pln Homing Receptor, CD62L Antigen, L-Selectin, CD62L, PLNHR, LAM-1, LAM1, LEU8, LSEL, L-selectin.
Sf9, Baculovirus cells.
ADPWTYHYSE KPMNWQRARR FCRDNYTDLV AIQNKAEIEY LEKTLPFSRS YYWIGIRKIG GIWTWVGTNK SLTEEAENWG DGEPNNKKNK EDCVEIYIKR NKDAGKWNDD ACHKLKAALC YTASCQPWSC SGHGECVEII NNYTCNCDVG YYGPQCQFVI QCEPLEAPEL GTMDCTHPLG NFSFSSQCAF SCSEGTNLTG IEETTCGPFG NWSSPEPTCQ VIQCEPLSAP DLGIMNCSHP LASFSFTSAC TFICSEGTEL IGKKKTICES SGIWSNPSPI CQKLDKSFSM IKEGDYNHHH HHH.
Product Science Overview
L-Selectin is expressed constitutively on a wide variety of leukocytes, including lymphocytes, monocytes, and neutrophils . The protein is composed of several domains:
- Lectin domain: This domain is responsible for binding to specific carbohydrate structures on the surface of endothelial cells.
- Epidermal growth factor (EGF)-like domain: This domain is involved in protein-protein interactions.
- Short consensus repeat (SCR) domains: These domains are involved in the regulation of the protein’s function.
Two forms of L-Selectin have been reported, arising from post-translational modifications. The lymphocyte form has an apparent molecular weight of 74 kDa, while the neutrophil form ranges from 90-100 kDa .
L-Selectin mediates the initial capture and rolling of leukocytes on the vascular endothelium, a critical step in the immune response. This process allows leukocytes to exit the bloodstream and migrate to sites of infection or injury . L-Selectin binds to specific carbohydrate ligands on endothelial cells, facilitating the adhesion and subsequent transmigration of leukocytes.
Recombinant L-Selectin is produced using various expression systems, including Sf9 insect cells. The recombinant protein produced in Sf9 cells is a single, glycosylated polypeptide chain containing 303 amino acids (52-345a.a.) and has a molecular mass of approximately 34.1 kDa . The molecular size on SDS-PAGE appears at approximately 40-57 kDa due to glycosylation .
Recombinant L-Selectin is used in various research applications, including:
- Cell adhesion studies: Understanding the mechanisms of leukocyte trafficking and adhesion.
- Immunological assays: Serving as a positive control in Western blotting, ELISA, and immunoprecipitation experiments .
- Drug development: Investigating potential therapeutic targets for inflammatory diseases and immune disorders.
